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Modified Organism
SPS-ØØJ55-2 - Innate™ Atlantic Potato
Record information and status
Record ID
109057
Status
Published
Date of creation
2015-10-19 18:09 UTC (dina.abdelhakim@cbd.int)
Date of last update
2015-10-19 18:26 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-10-19 18:26 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Innate™ Atlantic Potato
Transformation event
J55
Unique identifier
SPS-ØØJ55-2
Developer(s)
J.R. Simplot Company
5369 West Irving Street 
Boise, ID
United States of America, 83706
Phone:+1 (208) 780-6066
Description
Simplot Innate™ Potato is genetically engineered to silence genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Var. Atlantic
Related LMOs
SPS-ØØJ78-7 - Innate™ Atlantic Potato
Changes in quality and/or metabolite content - Pigmentation / Coloration, Protein and amino acids
SPS-ØØØJ3-4 - Innate™ Atlantic Potato
Changes in quality and/or metabolite content - Pigmentation / Coloration, Protein and amino acids
Characteristics of the transformation process
Vector
pSIM1278
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
This DNA insert comprises two expression cassettes and was inserted into the pSIM1278 transformation vector.

The first cassette comprises fragments of both the asparagine synthetase-1 gene (Asn1) and the polyphenol oxidase-5 gene (Ppo5), arranged as inverted repeats between the Agp promoter of the ADP  glucose pyrophosphorylase gene (Agp) and the Gbss promoter of the granule-bound starch synthase gene (Gbss) and results in silencing of both the Ppo5 and Asn1 genes.

The second cassette is comprised of fragments of the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL), operably linked to the same Agp and Gbss promoters as the first cassette. The function of the second cassette is to silence the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL).

Southern blot analysis indicated that event J55  contains 2 copies of the DNA insert, one of which is full and the other one lacking at least part of the R1/PhL promoter silencing cassette, and no vector backbone in the LMO.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records