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Modified Organism
SPS-000W8-4 - Innate™ Russet Burbank Potato
Record information and status
Record ID
109066
Status
Published
Date of creation
2015-10-21 15:11 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-10-21 15:11 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Innate™ Russet Burbank Potato
Transformation event
W8
Unique identifier
SPS-000W8-4
Developer(s)
J.R. Simplot Company
5369 West Irving Street 
Boise, ID
United States of America, 83706
Phone:+1 (208) 780-6066
Description
Simplot Innate™ Potato is genetically engineered to silence genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes. The potato was also transformed to express the late blight resistance gene to confer resistance to late blight disease.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Var. Russet Burbank
Characteristics of the transformation process
Vector
pSIM1678 and pSIM1278
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
 
Phytophthora infestans Resistance gene 1 Promoter
0.71 Kb
 
 
Phytophthora infestans Resistance gene 1
2.68 Kb
 
 
Phytophthora infestans Resistance gene 1 terminator
0.92 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Acid invertase gene
0.68 Kb
 
 
Acid invertase gene
0.68 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Genetic and structural characterization of the inserts associated with transformation of Russet Burbank by pSIM1278 and pSIM1678 to produce event W8 showed that both transformations resulted in a single integration site for each plasmid.

The structure of the DNA derived from transformation of pSIM1278 was complex relative to the structure of the original insert. The inserted DNA appears to have undergone rearrangement during transformation resulting in a structure consisting of a tandem repeat of the Asn1/Ppo5 silencing cassette, followed by a nearly complete pSIM1278 construct, and an inverted repeat containing a duplication of the pR1/pPhl silencing cassette and a tandem duplication of the Gbss promoter with intervening Phl sequence. Although this structure is more complicated than anticipated, the duplicated silencing cassettes are intact and remain under the control of the tissue-specific promoters.

W8 also contains a single copy of the DNA from pSIM1678 that resides at a single locus of integration. The DNA insert of pSIM1678 contains a nearly intact DNA insert with a 330-bp deletion, which removes the entire T-DNA left border and 137-bp of the Rpi-vnt1 promoter.

Southern blot and PCR analyses have shown that the Russet Burbank W8 event does not contain backbone from either plasmid used in the transformations.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents