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Modified Organism
Tobacco modified for antibiotic resistance and GFP expression
Record information and status
Record ID
109069
Status
Published
Date of creation
2015-10-21 11:44 UTC (german_bch@bvl.bund.de)
Date of publication
2015-10-21 16:37 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Tobacco modified for antibiotic resistance and GFP expression
Transformation event
Nt-pDK53
Developer(s)
Universität Rostock
Agrobiotechnology - Faculty of Agricultural and Environmental Sciences
Justus-von-Liebig-Weg 6

Rostock, Mecklenburg-Vorpommern
Germany, 18059
Phone:+49 381 498 ext 3080
Fax:+49 381 498 ext 3082
Email:dekan.auf@uni-rostock.de
Url:Rostock University,Agrobiotechnology - Rostock University
Description
Tobacco line Nt-pDK53 contains chloroplasts that were genetically modified to express the GFP reporter gene and aadA selection marker, to evaluate the pollen-mediated transfer of transgenes located in the plastid genome in cross-breeding partners of field grown tobacco.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Nicotiana tabacum - Tobacco, TOBAC
Point of collection or acquisition of the recipient organism
Cultivar: Petit Havana (male sterile line)
Related LMOs
Petunia modified for antibiotic resistance
Universität Rostock Resistance to antibiotics - Streptomycin Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pDK53
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
30S ribosomal protein S16 gene terminator
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Green Fluorescent Protein gene
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16S rRNA gene promoter
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Photosystem II protein D1 gene promoter
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3"(9)-O-aminoglycoside adenyltransferase
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D1 thylakoid membrane protein (psbA) gene terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The LMO Nt-pDK53 is a transplastomic tobacco line, therefore, chloroplast DNA was genetically modified to express the antibiotic resistance gene aadA from Escherichia coli, which is under the control of the promoter and terminator of the psbA gene, and the GFP gene from Aequoria victoria whereby expression is regulated by the 16S rRNA gene promoter, in combination with a synthetic Shine-Dalgarno sequence. The 30S ribosomal protein S16 gene terminator was used at the 3' end of the GFP coding sequence.

The transformation process was conducted in two steps:

First, the gfp reporter gene was directly linked to the aadA selectable marker gene  within the pDK53 vector to ensure cointegration of the two markers into the plastid genome by homologous recombination via the flanking chloroplast sequences.

Second, young leaves from sterile tobacco plants were bombarded with plasmid-coated, 0.6-μm gold particles by using a biolistic gun.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record