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Modified Organism
Sugar beet modified for herbicide tolerance
Record information and status
Record ID
109070
Status
Published
Date of creation
2015-10-21 08:42 UTC (german_bch@bvl.bund.de)
Date of last update
2020-03-26 15:27 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-03-26 15:27 UTC (austein.mcloughlin@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Sugar beet modified for herbicide tolerance
Transformation event
T227-1
Developer(s)
Monsanto Europe S.A.
Avenue de Tervuren 270-272
Brussels
Belgium, B-1150
Description
The T227-1 sugar beet (Beta vulgaris) was modified with the insertion of Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) to confer herbicide tolerance to the glyphosate. The variation in the bacterial enzyme allows the plant to tolerate otherwise lethal doses of glyphosate.

Note:
The beet lines T227-1 and H7 (Record ID: 14773 - see below) are independent transformation lines containing the same genetic elements.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Beta vulgaris - Common beet, Sugarbeet, BETMA
Related LMOs
KM-ØØØH71-4 - Roundup Ready™ sugar beet
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
pMON17227
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S promoter
0.67 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
rbcS-E9 gene terminator
0.64 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transcription of the Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene is under control of the Figwort Mosaic Virus 35S promoter and the Pisum sativum Rubisco small subunit (rbcS-E9) terminator. The transcript contains an Arabidopsis thaliana chloroplast transit peptide at the 5' end of the transcript to facilitate post-translational targeting to the chloroplast. Due to the viral promoter, transcription is expected to occur constitutively at elevated levels.

Please note: the nucleic acid sequence of the epsps gene was codon-optimized for expression in plants.
LMO characteristics
Modified traits
Common use(s)
  • Research
Additional Information
Additional Information
The sugar beet lines T227-1 and H7-1 are independent transformation events harbouring the same genetic elements. 
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record