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Modified Organism
Wheat modified for low celiac-toxic epitope content
Record information and status
Record ID
109214
Status
Published
Date of creation
2015-11-10 17:46 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-11-10 17:46 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Wheat modified for low celiac-toxic epitope content
Transformation event
E82
Developer(s)
Francisco Barro
Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Cientificas (CSIC)
Córdoba
Spain
Email:fbarro@ias.csic.es
Description
Wheat was modified using RNAi technology to down-regulate the expression of gliadins in bread wheat. A set of hairpin constructs were designed and expressed in the endosperm of bread wheat. The expression of gliadins was strongly down-regulated in the transgenic lines.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Triticum aestivum - Wheat
Characteristics of the transformation process
Vector
pDhp_omega_alpha and pAHC25
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Hor3-1 gene promoter
0.87 Kb
 
 
Chimeric wheat gliadin
0.36 Kb
 
 
Ubiquitin Intron 1
1.02 Kb
 
 
Chimeric wheat gliadin
0.36 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Beta-Glucuronidase coding sequence
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record