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Modified Organism
Pea modified for alpha-amylase expression
Record information and status
Record ID
109360
Status
Published
Date of creation
2015-11-24 10:23 UTC (german_bch@bvl.bund.de)
Date of publication
2015-11-24 17:12 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Pea modified for alpha-amylase expression
Transformation event
pGPTV-BAR::USPPAMYLI
Developer(s)
Institut für Pflanzengenetik und Kulturpflanzenforschung
Corrensstrasse 3 
Gatersleben
Germany, 06466
Phone:+49 (0)39482 5-0
Fax:+49 (0) 39482 5139
Email:info@ipk-gatersleben.de
Url:IPK Gatersleben
Description
Genetically modified pea plants in which the bacterial alpha-amylase gene is present in homozygous condition do not show any significant changes in configuration and general appearance. Nevertheless, it cannot be excluded that the effects of the recombinant alpha-amylase on the plant will become noticeable under field conditions. The recombinant alpha-amylase is located in protein bodies. However, the endogenous seed starch, the main constituent of pea seeds (approx. 70%), is assumed to be degraded also at suboptimal temperatures, resulting in the formation of mainly maltose, maltotriose and alpha-dextrin. The partial change in the carbohydrate composition may, amongst other things, change the osmotic value of the seeds and increase their frost resistance.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Pisum sativum - Garden pea, PEA
Point of collection or acquisition of the recipient organism
Cultivar: Erbi
Characteristics of the transformation process
Vector
pGPTV-BAR
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Unknown seed protein-promoter - Vicia faba - Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean
Alpha-amylase gene - Bacillus licheniformis - BACLI
Changes in quality and/or metabolite content - Carbohydrates
Octopine Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Phosphinothricin N-acetyltransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to herbicides - Glufosinate
Transcript 7 gene 3' untranslated region - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Beta-Glucuronidase coding sequence - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
Notes regarding the genetic elements introduced or modified in this LMO
The genetically modified pea plants contain 1800 bp of the 1948-bp AmyLi sequence (alpha-amylase gene) from Bacillus licheniformis that is controlled by the seed-specific USP promoter of broad beans (Vicia faba) and the terminator sequence of the ocs gene from A. tumefaciens.
In the genetically modified plants, the bar gene is controlled by the promoter of the nos gene and the terminator sequence of the g7 gene from A. tumefaciens. It was used for the selec-tion of transformed plant cells.
The uidA gene that codes for the enzyme beta-glucuronidase is promoterless and contains the nopaline synthase termination region of A. tumefaciens. It is a constituent of the Ti region of the transformation vector pGPTV-BAR. The uidA gene is not expected to be expressed in the genetically modified pea plants.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record