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Modified Organism
MON-87751-7 - Insect resistant soybean
Record information and status
Record ID
110062
Status
Published
Date of creation
2016-04-08 00:51 UTC (asela.atapattu@epa.govt.nz)
Date of publication
2016-04-12 20:12 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant soybean
Transformation event
MON87751
Unique identifier
MON-87751-7
Developer(s)
Monsanto
Description
Soybean was modified with the insertion of the Cry1A.105 and Cry2Ab2 proteins which provide protection from feeding damage caused by targeted lepidopteran pests, such as primary target pests velvetbean caterpillar (Anticarcia gemmatalis), soybean looper (Pseudoplusia includens), soybean anxil borer (Epinotia aporema), and sunflower looper (Rachiplusia nu).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Related LMOs
MON-877Ø1-2 - Insect resistant soybean
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GMIR13196
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Actin 2 Gene Promoter
1.21 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
Cry2Ab2
1.90 Kb
 
 
Metallothionein-like gene terminator
0.30 Kb
 
 
rbcS Promoter
1.72 Kb
 
 
rbcS Transit Peptide
0.26 Kb
 
 
Cry1A.105
3.53 Kb
 
 
Phosphate transporter gene terminator
0.40 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
PV-GMIR13196 contains two separate T-DNAs that are each delineated by left and right border regions. The first T-DNA, designated as T-DNA I, contains the crylA.105 and cry2Ab2 expression cassettes, with genetic elements as indicated above.

The second T-DNA, designated as T-DNA II, contains marker genes that allow for simplified selection of transformed tissue. During transformation, both T-DNAs were inserted into the soybean genome. Subsequently, traditional breeding, segregation, selection and screening were used to isolate those plants that contain the crylA.105 and cry2Ab2 expression cassettes and do not contain the marker gene expression cassettes.

Characterization of the DNA insert in MON 87751 was conducted using a combination of sequencing, PCR, and bioinformatics. The results of this characterization demonstrate that MON 87751 contains one copy of T-DNA I containing the cry1A.105 and cry2Ab2 expression cassettes that is stably integrated at a single locus and is inherited according to Mendelian principles over multiple generations. No vector backbone sequences were detetcted
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (7)
IDDescription
7record(s) found
Country's Decision or any other Communication3 records
Modified Organism1 record
Risk Assessment3 records