PV-GMIR13196 contains two separate T-DNAs that are each delineated
by left and right border regions. The first T-DNA, designated as
T-DNA I, contains the crylA.105 and cry2Ab2 expression cassettes,
with genetic elements as indicated above.
The second T-DNA, designated as T-DNA II, contains marker genes
that allow for simplified selection of transformed tissue. During
transformation, both T-DNAs were inserted into the soybean genome.
Subsequently, traditional breeding, segregation, selection and
screening were used to isolate those plants that contain the
crylA.105 and cry2Ab2 expression cassettes and do not contain the
marker gene expression cassettes.
Characterization of the DNA insert in MON 87751 was conducted using
a combination of sequencing, PCR, and bioinformatics. The results
of this characterization demonstrate that MON 87751 contains one
copy of T-DNA I containing the cry1A.105 and cry2Ab2 expression
cassettes that is stably integrated at a single locus and is
inherited according to Mendelian principles over multiple
generations. No vector backbone sequences were detetcted