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Modified Organism
Herbicide tolerant canola
Record information and status
Record ID
110263
Status
Published
Date of creation
2016-05-05 17:19 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-05-05 17:19 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant canola
Transformation event
CLB-1
Developer(s)
BASF Plant Science GmbH
Carl-Bosch-Str. 38
Ludwigshafen
Germany, 67056
Phone:+49 621 60-0
Fax:+49 621 60-42525
Email:global.info@basf.com
Url:BASF AG - Pflanzenbiotechnologie
Description
A canola (Brassica napus) event that is tolerant to the imidazolinone herbicides imazamox and imazapyr. Canola event CLB-1 was developed using site-directed mutagenesis of the AHAS3 gene in B. napus.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Characteristics of the transformation process
Techniques used for the modification
  • Site Directed Mutagenesis
Genetic elements construct
 
Acetohydroxy acid synthase 3 gene Promoter
0.00 Kb
 
 
Acetohydroxy acid synthase 3 gene
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Acetohydroxy acid synthase 3 gene terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Canola event CLB-1 was developed through sequential site-directed mutagenesis of the AHAS3 gene in canola cells. Mutated cells were selected on the basis of tolerance to imazapyr and regenerated to produce plants. Canola event CLB-1 was identified as a successful mutant based on molecular analyses, herbicide efficacy and agronomic evaluations and was thus chosen for further development.

This gene encodes an acetohydroxyacid synthase (AHAS) enzyme, which is also referred to as an acetolactate synthase (ALS) enzyme. The herbicide tolerance trait in canola event CLB-1 is conferred by two point mutations, an alanine to threonine substitution at position 122 and a serine to asparagine substitution at position 653, in the AHAS3 gene.

These mutations result in a modified AHAS3 enzyme carrying two amino acid substitutions which render the enzyme insensitive to imidazolinone herbicides, including imazamox and imazapyr.

The canola event CLB-1 imidazolinone herbicide tolerance trait is under the control of the native AHAS3 promoter and is believed to be constitutively expressed.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents