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Modified Organism
SYN-IR1Ø2-7 x MON-15985-7 x MON-88913-8 - Insect resistant, Herbicide tolerant Cotton
Record information and status
Record ID
110490
Status
Published
Date of creation
2016-05-23 20:36 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-05-23 20:36 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant, Herbicide tolerant Cotton
Transformation event
COT102 x MON15985 x MON88913
Unique identifier
SYN-IR1Ø2-7 x MON-15985-7 x MON-88913-8
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The stacked cotton line COT102 x MON15985 x MON88913 was obtained through the traditional cross breeding of each of the parental organisms to produce a plant that expresses the Vip3A, Cry1Ac and Cry2Ab proteins  which confer resistance to lepidopteran pests and the EPSPS (5-enolpyruvylshikimaete-3-phosphate synthase) that confers tolerance to the herbicide glyphosate. The aph4 gene, coding for hygromycin-B phosphotransferase (APH4) selectable marker is also present in the plant.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
MON-88913-8 - Roundup Ready™ Flex™ cotton
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-15985-7 - Bollgard II™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
SYN-IR1Ø2-7 - VIPCOT™ Cotton
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pCOT-1, PV-GHGT35, PV-GHBK04 and PV-GHBK11
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
FMV 35S Enhancer
0.00 Kb
 
 
Elongation factor EF-1alpha promoter
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Elongation factor EF-1alpha Leader
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Elongation factor EF-1alpha Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
CaMV 35S Enhancer
0.00 Kb
 
 
Actin 8 Promoter
0.00 Kb
 
 
Actin 8 Leader sequence
0.00 Kb
 
 
Actin 8 Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
HSP 70 5' untranslated leader sequence
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
Cry2Ab2
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Beta-Glucuronidase coding sequence
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene 3 promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Actin 2 Gene Promoter
0.00 Kb
 
 
Vegetative insecticidal protein 3A
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON 88913 vector PV-GHGT35:
Roundup Ready® Flex cotton (MON 88913) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in cotton production. This genetically engineered cotton contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 88913 to survive otherwise lethal applications of glyphosate. The EPSPS gene introduced into MON 88913 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the EPSPS enzyme expressed by this gene is tolerant to glyphosate. MON 88913 cotton contains two copies of the EPSPS gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage.

DNA insert from MON15985 vector PV-GHBK11 and PV-GHBK04:
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.

DNA insert from COT102, vector pCOT-1:
Cotton tolerant to lepidopteran pests through introduction of the vip3A(a) gene which codes for an insecticidal protein that targets lepidopteran insect species. COT102 cotton was developed as an alternative and novel insect control option for lepidopteran pests of cotton. COT102 was also developed to help prevent and manage resistance to Cry proteins due to the different insecticidal mode of action of VIP proteins.  The aph4 gene, coding for hygromycin-B phosphotransferase (APH4) was used as a selectable marker.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication2 records
Risk Assessment2 records