| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
SYN-ØØØJG-2 - Herbicide tolerant maize
Record information and status
Record ID
110613
Status
Published
Date of creation
2016-06-22 15:54 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-06-22 15:54 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant maize
Transformation event
MZHG0JG
Unique identifier
SYN-ØØØJG-2
Developer(s)
Syngenta Seeds GmbH
Syngenta Seeds GmbH
Zum Knipkenbach 20
Bad Salzuflen
Germany, 32107
Phone:+49 52 22 5308-0
Fax:+49 52 22 5308-12
Email:info.seeds@syngenta.com
Url:Syngenta Seeds Germany
Description
MZHG0JG corn was genetically modified to tolerate herbicides containing glyphosate and glufosinate-ammonium with the insertion of the transgene mepsps-02, which encodes the enzyme modified 5-enol pyruvylshikimate-3-phosphate synthase (mEPSPS), and the transgene pat-09, which encodes the enzyme phosphinothricin acetyltransferase (PAT).

Note: SYN-ØØØJG-2, is also referred to with the UID SYN-ØØØJG-3.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
pSYN18857
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S Enhancer
0.10 Kb
 
 
CaMV 35S Enhancer
0.29 Kb
 
 
Ubiquitin gene promoter
3.99 Kb
 
 
Omega 5' untranslated leader
0.07 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
Double Mutant 5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Ubiquitin gene terminator
1.00 Kb
 
 
CaMV 35S promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Genetic characterization studies demonstrated that MZHG0JG corn contains, at a single locus within the corn genome, a single copy of each of the following functional elements: mepsps-02, pat-09, FMV-05 enhancer, 35S-05 enhancer, OTP-02 transit peptide, Ubi158-02 promoter, TMV-03 enhancer, Ubi158-02 terminator, 35S-19 promoter, and NOS-05-01 terminator. It does not contain any extraneous DNA fragments of these functional elements elsewhere in the MZHG0JG corn genome, and it does not contain the plasmid backbone sequence from transformation plasmid pSYN18857.

Nucleotide sequence analysis determined that the MZHG0JG insert consists of the intact T-DNA region of the pSYN18857 plasmid vector. The results of the Southern blot analyses are consistent with the results of the nucleotide sequence analysis.

Sequence analysis of the MZHG0JG insertion site demonstrated that 22 bp from the corn genomic sequence were deleted during the integration of the MZHG0JG insert, and 43 bp of DNA were inserted into the integration site; a 4-bp DNA sequence was present at the junction between the MZHG0JG insert and the 5ʹ flanking region and a 39-bp DNA sequence was present at the junction between the MZHG0JG insert and the 3ʹ flanking region. BLASTN analyses comparing the corn genomic sequence flanking the MZHG0JG insert with sequences in public databases indicated that the insert does not disrupt any known endogenous corn gene.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record