Genetic characterization studies demonstrated that MZHG0JG corn
contains, at a single locus within the corn genome, a single copy
of each of the following functional elements: mepsps-02, pat-09,
FMV-05 enhancer, 35S-05 enhancer, OTP-02 transit peptide, Ubi158-02
promoter, TMV-03 enhancer, Ubi158-02 terminator, 35S-19 promoter,
and NOS-05-01 terminator. It does not contain any extraneous DNA
fragments of these functional elements elsewhere in the MZHG0JG
corn genome, and it does not contain the plasmid backbone sequence
from transformation plasmid pSYN18857.
Nucleotide sequence analysis determined that the MZHG0JG insert
consists of the intact T-DNA region of the pSYN18857 plasmid
vector. The results of the Southern blot analyses are consistent
with the results of the nucleotide sequence analysis.
Sequence analysis of the MZHG0JG insertion site demonstrated that
22 bp from the corn genomic sequence were deleted during the
integration of the MZHG0JG insert, and 43 bp of DNA were inserted
into the integration site; a 4-bp DNA sequence was present at the
junction between the MZHG0JG insert and the 5ʹ flanking region and
a 39-bp DNA sequence was present at the junction between the
MZHG0JG insert and the 3ʹ flanking region. BLASTN analyses
comparing the corn genomic sequence flanking the MZHG0JG insert
with sequences in public databases indicated that the insert does
not disrupt any known endogenous corn gene.