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Modified Organism
Black nightshade modified for reduced production of endogenous proteinase inhibitors
Record information and status
Record ID
110660
Status
Published
Date of creation
2016-07-04 10:50 UTC (german_bch@bvl.bund.de)
Date of last update
2016-07-20 21:47 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-07-20 21:47 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Black nightshade modified for reduced production of endogenous proteinase inhibitors
Transformation event
S06-336, S06-353, S06-354 and S06-356
Developer(s)
Max-Planck-Institut für Chemische Ökologie
Hans-Knöll-Strasse 8
D-07745 Jena
Germany
Description
The genes pin1 and pin2b from Solanum nigrum each codes for a type II  serine proteinase inhibitors (PIN), which block the proteolytic activity of trypsin and chymotrypsin. Plants produce proteinase inhibitors as a strategy to protect themselves against the damage caused by herbivorous insects.

In the genetically modified plants S06-336, S06-353, S06-354 and S06-356 the synthesis of two of the plant's native proteinase inhibitors, PIN1 and PIN2b, is reduced by post-transcriptional gene silencing. For this purpose, an RNAi construct consisting of fragments of the pin1 and pin2b genes  from S. nigrum was developed.

As a result of the genetic modification, the targeted reduction of the production of the PIN2b and PIN1 proteins is expected to reduce the genetically modified plant's defences against herbivory.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum nigrum - Black nightshade, SOLNI
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Sn 30
Related LMOs
Black Nightshade with reduced pathogenesis-related protein 1S synthesis.
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Black Nightshade with reduced pathogenesis-related protein 1S synthesis
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Black nightshade modified for reduced production of endogenous prosystemin
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pSOL3PIN12
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S terminator
0.00 Kb
 
 
Potato type II proteinase inhibitor, PINI
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Potato type II proteinase inhibitor, PIN2B
0.00 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.00 Kb
 
 
Potato type II proteinase inhibitor, PIN2B
0.00 Kb
 
 
Potato type II proteinase inhibitor, PINI
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Fragments of the proteinase inhibitor genes pin1 and pin2b derived from S. nigrum were linked in a chimeric fragment of 728 bp. The plasmid pSOL3-PIN12 used for transformation includes a construct in which two of these 728 bp fragments were arranged in sense and antisense orientation, separated by a spacer. The 785 bp long third intron of the pyruvate orthophosphate dikinase gene (pdk i3) of Flaveria trinervia, of which splicing activity is suspected, was used as a spacer. The expression is controlled by the promoter and the termination signal of the 35S gene of cauliflower mosaic virus (CaMV).

The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and termination signal of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication1 record
Modified Organism3 records
Risk Assessment1 record