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Modified Organism
Black nightshade modified for reduced production of endogenous prosystemin
Record information and status
Record ID
110661
Status
Published
Date of creation
2016-07-04 10:50 UTC (german_bch@bvl.bund.de)
Date of last update
2016-07-21 16:24 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-07-21 16:24 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Black nightshade modified for reduced production of endogenous prosystemin
Transformation event
S03/71; S03/82
Developer(s)
Max-Planck-Institut für Chemische Ökologie
Hans-Knöll-Strasse 8
D-07745 Jena
Germany
Description
Black nightshade was modified to reduce the synthesis of the plant's native prosystemin  by post-transcriptional gene silencing using an RNAi construct which was developed from fragments of the prosystemin gene from S. nigrum

The prosystemin gene codes for the 200 amino acid prosystemin peptide, which is the precursor of the active 18 amino acid systemin peptide. Systemin is a signaling molecule which plays an important role in mediating the systemic wound response in plant tissues.

The modification resulted in the targeted reduction of the production of the prosystemin peptide leading to a decrease in the plant's defences against herbivores/pathogen infestation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum nigrum - Black nightshade, SOLNI
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Sn 30
Related LMOs
Black nightshade modified for reduced production of endogenous proteinase inhibitors
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects Selectable marker genes and reporter genes
Black Nightshade with reduced pathogenesis-related protein 1S synthesis
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Black Nightshade with reduced pathogenesis-related protein 1S synthesis.
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pSOL3SYS
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
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Prosystemin gene
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Pyruvate orthophosphate dikinase, Intron 3
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Prosystemin gene
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CaMV 35S promoter
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Nopaline Synthase Gene Promoter
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Hygromycin B phosphotransferase gene
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The plasmid pSOL3SYS used for transformation includes a construct in which two complementary 404 bp fragments of the prosystemin gene (nigpro) derived from S. nigrum were arranged in sense and antisense orientation, separated by a spacer. The 785 bp long third intron of the pyruvate orthophosphate dikinase gene (pdk i3) of Flaveria trinervia (Asteraceae), of which splicing activity is suspected, was used as a spacer. The expression is controlled by the promoter and the termination signal of the 35S gene of cauliflower mosaic virus (CaMV).

The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and termination signal of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
Modified traits
  • Resistance to diseases and pests
Common use(s)
  • Research
Additional Information

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication1 record
Modified Organism3 records
Risk Assessment1 record