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Record details
Modified Organism
Black nightshade modified for reduced production of endogenous prosystemin
LMO Information
Decisions on the LMO
Risk Assessments
Record information and status
Record ID
110661
Status
Published
Date of creation
2016-07-04 10:50 UTC (german_bch@bvl.bund.de)
Date of last update
2016-07-21 16:24 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-07-21 16:24 UTC (dina.abdelhakim@cbd.int)
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Living Modified Organism identity
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LMO quick-links
page.
LMO name
Black nightshade modified for reduced production of endogenous prosystemin
Transformation event
S03/71; S03/82
Developer(s)
Record #48031
Max-Planck-Institut für Chemische Ökologie
Hans-Knöll-Strasse 8
D-07745 Jena
Germany
Description
Black nightshade was modified to reduce the synthesis of the plant's native prosystemin by post-transcriptional gene silencing using an RNAi construct which was developed from fragments of the prosystemin gene from S. nigrum
The prosystemin gene codes for the 200 amino acid prosystemin peptide, which is the precursor of the active 18 amino acid systemin peptide. Systemin is a signaling molecule which plays an important role in mediating the systemic wound response in plant tissues.
The modification resulted in the targeted reduction of the production of the prosystemin peptide leading to a decrease in the plant's defences against herbivores/pathogen infestation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Record #47832
Solanum nigrum - Black nightshade, SOLNI
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Sn 30
Related LMOs
Record #110660
Black nightshade modified for reduced production of endogenous proteinase inhibitors
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects Selectable marker genes and reporter genes
Record #108714
Black Nightshade with reduced pathogenesis-related protein 1S synthesis
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Record #108713
Black Nightshade with reduced pathogenesis-related protein 1S synthesis.
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pSOL3SYS
Techniques used for the modification
Agrobacterium-mediated DNA transfer
Genetic elements construct
CaMV 35S promoter
#100287
0.00 Kb
Prosystemin gene
#110670
0.00 Kb
Pyruvate orthophosphate dikinase, Intron 3
#103123
0.00 Kb
Prosystemin gene
#110670
0.00 Kb
CaMV 35S promoter
#100287
0.00 Kb
Nopaline Synthase Gene Promoter
#100270
0.00 Kb
Hygromycin B phosphotransferase gene
#14991
0.00 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The plasmid pSOL3SYS used for transformation includes a construct in which two complementary 404 bp fragments of the prosystemin gene (nigpro) derived from S. nigrum were arranged in sense and antisense orientation, separated by a spacer. The 785 bp long third intron of the pyruvate orthophosphate dikinase gene (pdk i3) of Flaveria trinervia (Asteraceae), of which splicing activity is suspected, was used as a spacer. The expression is controlled by the promoter and the termination signal of the 35S gene of cauliflower mosaic virus (CaMV).
The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and termination signal of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
Modified traits
Resistance to antibiotics
Hygromycin
Resistance to diseases and pests
Common use(s)
Research
Additional Information
Other relevant website address or attached documents
Solanum nigrum: a model ecological expression system and its tools
B/DE/07/0188 - SNIF
Records referencing this document
(
5
)
ID
Description
5
record(s) found
Country's Decision or any other Communication
1 record
Modified Organism
3 records
Risk Assessment
1 record
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