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Modified Organism
Potato modified for increased oxygen supply to tubers
Record information and status
Record ID
110680
Status
Published
Date of creation
2016-07-13 10:03 UTC (german_bch@bvl.bund.de)
Date of publication
2016-07-24 18:59 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for increased oxygen supply to tubers
Transformation event
B33-LegHg-3‘OCS 13; B33-LegHg-3‘OCS 45; B33-LegHg-3‘OCS 54 and B33-LegHg-3‘OCS 57
Developer(s)
Max Planck Institute of Molecular Plant Physiology (MPIMP)
Max-Planck-Institut für Molekulare Pflanzenphysiologie
Am Mühlenberg 1
Potsdam
Germany, 14476
Phone:+49 331 567 - 80
Fax:+49 331 567 - 84 08
Email:contact@mpimp-golm.mpg.de
Url:Max-Planck-Institut für Molekulare Pflanzenphysiologie
Description
The potato plants were genetically modified to express the Leghemoglobin gene from [i[Lotus japonicus[/i] under the control of a tuber-specific promoter.

The genetic modification is expected to lead to increased oxygen content in the tuber at up to a depth of 2 mm below the skin, leading to enlarged lenticels and an increased tuber starch content.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Desirée
Characteristics of the transformation process
Vector
Derivative of pART27 (B33-LegHg-3'OCS)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
B33 gene promotor
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Leghaemoglobin gene
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Octopine Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The papatin-B33 promoter from Solanum tuberosum mediates a tissue-specific expression of the leghaemoglobin gene in the tubers. The ocs terminator from Agrobacterium tumefaciens is used as a termination signal.

The nptII gene from Escherichia coli is expressed under the control of the promoter and terminator signal of the nopaline synthase gene from Agrobacterium tumefaciens and it was used as a selection marker.

The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
Modified traits
Common use(s)
  • Research
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record