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Modified Organism
Potato modified for increased yield and tuber starch content.
Record information and status
Record ID
110729
Status
Published
Date of creation
2016-07-05 06:59 UTC (german_bch@bvl.bund.de)
Date of publication
2016-08-01 20:46 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
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LMO name
Potato modified for increased yield and tuber starch content.
Transformation event
BG1BA-24, BG1BA-31 and BG1BA-32
Developer(s)
Uni Köln
Botanisches Institut
Universität zu Köln
Albert-Magnus-Platz
Köln, Nordrhein-Westfalen
Germany, 50923
Phone:+ 49 221 470-2473
Fax:+ 49 221 470-5062
Email:verwaltungspoststelle@verw.uni-koeln.de
Url:http://www.botanik.uni-koeln.de/
Description
In the modified potato lines BG1BA-24, BG1BA-31 and BG1BA-32 were engineered to tissue-specifically express the gpt-gene, from P. sativum, and the ntt-gene, from [i]A. thaliana,[/]  in the potato tubers, with the possibility of sucrose induced expression in the shoot region.

The gpt gene from pea (Pisum sativum) codes for a plastid glucose-6-phosphate/phosphate translocator, an antiporter whose main physiological function is to import glucose-6-phosphate, in exchange for anorganic phosphate, into the plastids of heterotrophic tissue. Here, it is responsible for the energy-dependent synthesis of starch, using glucose-6-phosphate as a precursor.

The ntt gene from Arabidopsis thaliana codes for a plastid nucleotide translocator. This is also an antiporter which catalyses the plastid uptake of ATP in exchange for ADP and is consequently also referred to as the ATP/ADP translocator. Its main function is to supply non-green storage plastids with ATP, which is needed there for starch synthesis.

As a result of the genetic modification, an increase in the transport activities of glucose-6-phosphate and ATP is expected, which in turn results in an increased tuber yield as well as an increase in the starch content in the tubers of the genetically modified potato plants.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Désirée
Characteristics of the transformation process
Vector
pBinB33(Hyg)::GPT: and pBinB33(Kan)::NTT:
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
B33 gene promotor
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Glucose-6-phosphate/phosphate-translocator gene
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Octopine Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Hygromycin B phosphotransferase gene
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Transcript 7 gene 3' untranslated region
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B33 gene promotor
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Plastidic nucleotide transporter gene
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Octopine Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Transcript 7 gene 3' untranslated region
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The potato plants described here are transformed with two different constructs:

pBinB33(Hyg)::GPT:
The plasmid  pBinB33(Hyg)::GPT: used for transformation includes a GPT-expression construct consisting of the B33-promotor of the patatin class I gene from Solanum tuberosum, the glucose-6-phosphate/phosphate-translokator (gpt)-gene from Pisum sativum and the ocs-terminator from Agrobacterium tumefaciens. The hygromycin phosphotransferase gene (hpt) of Streptomyces hygroscopicus expressed under the control of the nos-promotor of the pTiT37 plasmid from A. tumefaciens and the terminator of the gene 7 from A. tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.

pBinB33(Kan)::NTT:
The plasmid pBinB33(Kan)::NTT: used for transformation includes a NTT-expression construct consisting of the B33-promotor of the patatin class I gene from S. tuberosum, the nukleotide-translokator (ntt)-gene from Arabidopsis thaliana and the ocs-terminator from A. tumefaciens. A part of the ornithin-cyclo-deaminase (ocd)-gene from A. tumefaciens in front of the neomycin phosphotransferase II gene (nptII) of the Tn5-Transposon of E. coli expressed under the control of the nos-promotor of the pTiT37 plasmid from A. tumefaciens and the terminator of the gene 7 from A. tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
Modified traits
Common use(s)
  • Research
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
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2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record