Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus | BCH-LMO-SCBD-110732 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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BCH-LMO-SCBD-110732-1   |   PDF   |   Print   |  
Decisions on the LMO Risk Assessments  
last updated: 26 Jul 2016
Living Modified Organism identity
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Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus
EN
T 210-3
No
The genetically modified sugarbeets was modified to constitutively express the coat protein gene of the Beet Necrotic Yellow Vein Virus (BNYVV), an important domain for encapsidation, vector transmissibility and cell-to-cell transmission.

The constitutively expressed capsid protein gene gives rise to a concentration of coat protein in every cell of the genetically modified plant tissue which is far below the concentration found in plants infected with BNYVV.

As a result of the genetic modification a BNYVV resistance of the modified plants is expected.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus
    | PLANTA Angewandte Pflanzengenetik und Biotechnologie GmbH | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Viruses, Beet necrotic yellow virus (BNYV))
Characteristics of the modification process
Derivative of pBIN19
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-110730-1 BNYVV coat protein | Beet necrotic yellow vein virus (BNYVV)
    Protein coding sequence | Resistance to diseases and pests (Viruses, Beet necrotic yellow virus (BNYV))
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The cDNA of the coat protein gene of the Beet Necrotic Yellow Vein Virus (BNYVV) is expressed under the control of the double 35S promoter of the Cauliflower mosaic virus (CaMV). The CaMV 35S-terminator is used as a termination signal.

The neomycin phosphotransferase gene (nptII), expressed under the control of the promoter and termination signal of the nopaline synthase gene (nos) of Agrobacterium tumefaciens, is used as a selection marker.

The introduced nucleic acid is integrated in the genome of the recipient organism at more than 8 insertion sites.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
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