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Modified Organism
Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus
Record information and status
Record ID
110733
Status
Published
Date of creation
2016-07-26 10:51 UTC (german_bch@bvl.bund.de)
Date of publication
2016-08-01 23:05 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus
Transformation event
T 219-5
Developer(s)
PLANTA
PLANTA Angewandte Pflanzengenetik und Biotechnologie GmbH
Grimsehlstraße 31
Einbeck
Germany, 37574
Phone:+49 5561-3110
Fax:+49 5561-311243
Email:info@kws.de
Url:http://www.kws.de
Description
The genetically modified sugarbeets were modified to constitutively express the coat protein gene of the Beet Necrotic Yellow Vein Virus (BNYVV), an important domain for encapsidation, vector transmissibility and cell-to-cell transmission.

The constitutively expressed capsid protein gene gives rise to a concentration of coat protein in every cell of the genetically modified plant tissue which is far below the concentration found in plants infected with BNYVV. The gus gene was inserted for the histological determination of a successful transformation.

As a result of the genetic modification a BNYVV resistance of the modified plants is expected.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Beta vulgaris - Common beet, Sugarbeet, BETMA
Related LMOs
Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus
PLANTA Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Viruses - Beet necrotic yellow virus (BNYV)
Characteristics of the transformation process
Vector
Derivative of pBI121
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
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BNYVV coat protein
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CaMV 35S terminator
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CaMV 35S promoter
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Beta-Glucuronidase coding sequence
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Light inducible tissue-specific ST-LS1 gene
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Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The cDNA of the coat protein gene of the Beet Necrotic Yellow Vein Virus (BNYVV) is expressed under the control of the double 35S promoter of the Cauliflower mosaic virus (CaMV). The CaMV 35S-terminator is used as a termination signal.

Additionally, the gus-gene from Escherichai coli, in which an intron of the ST-LS1 gene from Solanum tuberosum was inserted, is expressed under the control of the CaMV 35S promoter. The terminator region of the nopaline synthase gene (nos) from A. tumefaciens is used as a termination signal.

The neomycin phosphotransferase gene (nptII), expressed under the control of the promoter and termination signal of the nopaline synthase gene (nos) of Agrobacterium tumefaciens, is used as a selection marker.

The introduced nucleic acid is integrated in the genome of the recipient organism at 7 - 9 insertion sites.
LMO characteristics
Modified traits
Common use(s)
  • Research
Additional Information
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record