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Modified Organism
Hybrid aspen modified for observing horizontal gene transfer into ectomycorrhizal fungi
Record information and status
Record ID
110856
Status
Published
Date of creation
2016-08-25 11:08 UTC (german_bch@bvl.bund.de)
Date of publication
2016-08-29 21:31 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Hybrid aspen modified for observing horizontal gene transfer into ectomycorrhizal fungi
Transformation event
Independent transformation events: T89-35S-Bar1, T89-35S-Bar3, T89-35S-Bar7, T89-35S-Bar12 and T89-35S-Bar14
Developer(s)
BFH
Federal Research Centre for Forestry and Forest Products (BFH)
Institute of Wood Research
Leuschnerstraße 91
21031 Hamburg-Bergedorf

Institute of Forest Genetics
Sieker Landstraße 2
22927 Großhansdorf

Johann Heinrich von Thünen Institute
Federal Research Institute for Rural Areas, Forestry and Fisheries
Bundesallee 50
Brunschweig
Germany, 38116
Phone:+49 531 596 1003
Fax:+ 49 531 596 1099
Email:info@thuenen.de
Url:https://www.thuenen.de/en/about-us/history/bfh-forestry-and-forest-products/
Description
The bar gene from Streptomyces hygroscopicus codes for a phosphinothricin acetyltransferase (PAT) which converts L-Phosphinothricin, the active component of the herbicidal agent glufosinate-ammonium, into the derivative N-acetyl-phosphinothricin which has no herbicidal effect.

In this trial the bar gene is to be used as a marker for evidence of horizontal gene transfer from GM hybrid aspen to ectomycorrhizal fungi. This does not require the development of herbicide tolerance in the hybrid aspen. For this reason, a construct was developed which expresses the native bar gene under the control of two different promoters. A fungal promoter is located at the 5' end of the bar gene. This is meant to allow for the selection of transgenic ectomycorrhizal fungi resulting from horizontal gene transfer. The 35S promoter of the cauliflower mosaic virus (CaMV) is positioned at the 3' end of the bar gene in an antisense orientation. This approach is meant to prevent the development of herbicide tolerance in the GM hybrid aspen in the event that the fungal promoter is also active in plants.

Therefore, as a result of the genetic modification, herbicide resistance of the hybrid aspen is not expected. After a successful horizontal gene transfer into ectomycorrhizal fungi, however, the bar gene should be expressed and the PAT enzyme should be formed, resulting in herbicide tolerant ectomycorrhizal fungi.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Populus tremula x Populus tremuloides - Hybrid aspen
Characteristics of the transformation process
Vector
pBI121-Bar
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Glyceraldehyde 3-phosphate dehydrogenase promoter
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Phosphinothricin N-acetyltransferase gene
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CaMV 35S promoter
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The bar gene from Streptomyces hygroscopicus coding for a phosphinothricin-acetyltransferaseis expressed under the control of two differnt promoters: the Glycerinaldehyd-3-phosphat-Dehydrogenase (GPD) promoter from Cochliobolus heterostrophus is located at the 5´ end of the gene for a transcription of the bar gene in sense orientation, the 35S promoter of the Cauliflower Mosaic Virus (CaMV) is located at the 3´ end of the gene for a transcription of the bar gene in antisense orientation.

The nptII gene coding for the neomycin phosphotransferase of the Tn5 transposon is used as a selection marker. It is expressed under the control of the promotor and the termination signal of the nopaline synthase gene (nos) Agrobacterium tumefaciens.

The transferred DNA is integrated into the genome of the recipient organism.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record