Potato modified for altered carbohydrate metabolism | BCH-LMO-SCBD-110885 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 06 Sep 2016
Living Modified Organism identity
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Potato modified for altered carbohydrate metabolism
EN
Multiple events: B33::cwiso-5, B33::cwiso-12 and B33::cwiso-26
No
Isomaltulose (also marketed as palatinose, 6-O-α-D-glucopyranosyl-D-fructose) is an isomer of sucrose. It can be synthesised by the sucrose isomerase gene palI from Erwinia rhapontici which converts sucrose mainly into its isomaltulose isomer.

Potato plants were modified to insert  a chimeric sucrose isomerase gene from Erwinia rhapontici under the control of a tuber-specific promoter. As a result of the genetic modification a change in the carbohydrate metabolism of the potato tubers is expected in such a way that isomaltulose is synthesised in the potato tubers while the sucrose concentration is decreased.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pBINB33::cwiso
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-110883-1 Proteinase inhibitor II transit peptide | Solanum tuberosum (Potato, SOLTU)
    Transit signal
  • BCH-GENE-SCBD-110884-1 Sucrose isomerase gene | Erwinia rhapontici (ERWRD)
    Protein coding sequence | Changes in quality and/or metabolite content (Carbohydrates)
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The transit peptide of the proteinase inhibitor II from Solanum tuberosum is fused to the N-terminus of the palI gene. The signal peptide of the proteinase inhibitor II  governs secretion of the enzyme into the apoplasmic space.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1