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Living Modified Organism (LMO)
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Potato modified for altered carbohydrate metabolism
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Multiple events: B33::cwiso-5, B33::cwiso-12 and B33::cwiso-26
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Organization:Institut für Pflanzengenetik und Kulturpflanzenforschung ()Corrensstrasse 3Gatersleben,
06466 , GermanyPhone: +49 (0)39482 5-0 ,Fax: +49 (0) 39482 5139,Email: info@ipk-gatersleben.de,Website: http://www.ipk-gatersleben.de,
Isomaltulose (also marketed as palatinose, 6-O-α-D-glucopyranosyl-D-fructose) is an isomer of sucrose. It can be synthesised by the sucrose isomerase gene palI from Erwinia rhapontici which converts sucrose mainly into its isomaltulose isomer.
Potato plants were modified to insert a chimeric sucrose isomerase gene from Erwinia rhapontici under the control of a tuber-specific promoter. As a result of the genetic modification a change in the carbohydrate metabolism of the potato tubers is expected in such a way that isomaltulose is synthesised in the potato tubers while the sucrose concentration is decreased.
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Potato plants were modified to insert a chimeric sucrose isomerase gene from Erwinia rhapontici under the control of a tuber-specific promoter. As a result of the genetic modification a change in the carbohydrate metabolism of the potato tubers is expected in such a way that isomaltulose is synthesised in the potato tubers while the sucrose concentration is decreased.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
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pBINB33::cwiso
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-110883-1 Proteinase inhibitor II transit peptide | Solanum tuberosum (Potato, SOLTU)Transit signal
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BCH-GENE-SCBD-110884-1 Sucrose isomerase gene | Erwinia rhapontici (ERWRD)Protein coding sequence | Changes in quality and/or metabolite content (Carbohydrates)
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
The transit peptide of the proteinase inhibitor II from Solanum tuberosum is fused to the N-terminus of the palI gene. The signal peptide of the proteinase inhibitor II governs secretion of the enzyme into the apoplasmic space.
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- Research
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