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Modified Organism
Oilseed rape modified for an altered fatty acid metabolism in the seeds
Record information and status
Record ID
110915
Status
Published
Date of creation
2016-09-12 10:36 UTC (german_bch@bvl.bund.de)
Date of publication
2016-09-20 16:26 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Oilseed rape modified for an altered fatty acid metabolism in the seeds
Transformation event
Independent lines transformed with one of pHS124, pHS126, or pHS127
Developer(s)
DSV
Deutsche Saatveredelung AG (DSV)
Weissenburger Straße 5
59557 Lippstadt
Lippstadt
Germany, 59557
Phone:+49 2941 2960
Fax:+49 2941 296100
Email:info@dsv-saaten.de
Url:https://www.dsv-saaten.de/
Description
Subgenic domains of an oleate desaturase gene (E12) from Brassica napus in expresses in the anti-sense orientation leading to the inhibition of the desaturation of oleic acid (C18:1) to linoleic acid (C18:2) in the genetically modified oilseed rape plants.

As a result, the oleic acid content is increased in the seeds, while the linoleic and linolenic acid content is reduced.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
Brassica napus modified for altered fatty acid metabolism
BAZ Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Oilseed rape modified for an altered fatty acid metabolism in the seeds
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Oilseed rape modified for an altered fatty acid metabolism in the seeds
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Oilseed rape modified for an altered fatty acid metabolism in the seeds
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Oilseed rape modified for an altered fatty acid metabolism in the seeds
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Oilseed rape modified for an altered fatty acid metabolism in the seeds
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Oilseed rape modified for an altered fatty acid metabolism in the seeds
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Oilseed rape modified for an altered fatty acid metabolism in the seeds
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Characteristics of the transformation process
Vector
pHS124, pHS126, or pHS127
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Acyl-acyl carrier protein thioesterase promoter
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Fatty acid deasturase 2 gene
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Acyl-acyl carrier protein thioesterase Terminator
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CaMV 35S promoter
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Neomycin Phosphotransferase II
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CaMV 35S terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Three constructs containing 400 - 700 bp fragments the oleate desaturase gene from Brassica napus in an antisense orientation were produced.

All three fragments are expressed under the control of the promoter and the terminator signal of the Acyl-[ACP] thioesterase gene from Cuphea lanceolata.

Each transgenic line originated from a transformation with one of the three constructs.

The neomycin phosphotransferase gene (nptII) from the Tn5 transposon expressed under the control of the 35S promoter and termination signal of the Cauliflower Mosaic Virus (CaMV) was used as a selection marker.

The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (10)
IDDescription
10record(s) found
Country's Decision or any other Communication1 record
Modified Organism8 records
Risk Assessment1 record