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Modified Organism
Red Clover modified for cold tolerance and fungal resistance
Record information and status
Record ID
Date of creation
2016-09-26 10:37 UTC (g.mozgova@yandex.ru)
Date of publication
2016-09-26 20:12 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Red Clover modified for cold tolerance and fungal resistance
Transformation event
Dr Tatiana Fomenko
Leading research associate
Laboratory of Cell Biotechnology
State Scientific Institution «Central Botanical Garden of the National Academy of Sciences of Belarus»
Surganova 2b
Belarus, 20012
Phone:+37517 284-14-84
Red clover, Trifolium pratense, modified with the insertion of the desA target gene, that encodes Δ12-desaturase of cyanobacteria.

Expression of Acyl-lipid ∆12-desaturase in the transgenic plants increases their tolerance to prolonged exposure to low temperatures and resistance to fungal pathogens and wounding.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Trifolium pratense - Red clover, Rotklee, Purple clover, Peavine clover, TRIPR
Point of collection or acquisition of the recipient organism
Variety: vitebchanin

Department of Genetics and Biotechnology of the Republican Unitary Enterprise «Research and Practical Center of the National Academy of Sciences of the Republic of Belarus for Arable Farming»
Characteristics of the transformation process
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
CaMV 35S promoter
0.00 Kb
Acyl-lipid ∆12-desaturase coding sequence
0.00 Kb
Lichenase coding sequence
0.00 Kb
ORF25 PolyA Terminator sequence
0.00 Kb
Nopaline Synthase Gene Promoter
0.00 Kb
Neomycin Phosphotransferase II
0.00 Kb
ORF25 PolyA Terminator sequence
0.00 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Target desA gene is fused with the sequence of the licBM3 reporter gene, that encodes lichenase.

The sequences of each of the genetic elements in the desА-licBM3 construct were not changed. 
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (1)
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