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Modified Organism
Oilseed rape modified for increased myristic acid content in the seeds
Record information and status
Record ID
110940
Status
Published
Date of creation
2016-09-27 09:21 UTC (german_bch@bvl.bund.de)
Date of publication
2016-09-29 16:56 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Oilseed rape modified for increased myristic acid content in the seeds
Transformation event
NBM99-ClFatB4 x NAT4; NBM99-EnClFatB4 x NAT4
Developer(s)
DSV
Deutsche Saatveredelung AG (DSV)
Weissenburger Straße 5
59557 Lippstadt
Lippstadt
Germany, 59557
Phone:+49 2941 2960
Fax:+49 2941 296100
Email:info@dsv-saaten.de
Url:https://www.dsv-saaten.de/
Description
A stacked oilseed rape was produced by crossing plsC expressing plants (transformed with pNAT4) and ClFatB4 expressing plants (transformed with pNBM99-ClFatB4 or pNBM99-EnClFatB4).

As a result of the genetic modification the seeds contain up to 10% myristic acid (C14:0), which is not normally present in rapeseed oil, and increased levels of palmitic acid (C16:0).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus modified for altered fatty acid metabolism
BAZ Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Brassica napus modified for altered fatty acid metabolism
DSV Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Kanamycin
Characteristics of the transformation process
Vector
pNAT4, and pNBM99-ClFatB4 or pNBM99-ENClFatB4
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Napin gene promoter
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1-acyl-sn-glycerol-3-phosphate acyltransferase coding sequence
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Napin gene terminator
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Acyl-acyl carrier protein thioesterase promoter
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Acyl-acyl carrier protein thioesterase ClFatB4
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Acyl-acyl carrier protein thioesterase Terminator
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CaMV 35S promoter
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Neomycin Phosphotransferase II
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CaMV 35S terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from vector pNAT4
The plsC gene from Escherichia coli encodes a lysophosphatidic acid acyltransferase (LPAAT) which catalyses the linkage of medium-chain fatty acids to the sn-2 position of lysophosphatidic acid. In the genetically modified oilseed rape plants transformed with pNAT4, LPAAT is expressed under the control of a seed specific promoter.

DNA insert from vector pNBM99-ClFatB4 and pNBM99-EnClFatB4
Oilseed rape plants  which were transformed with either of pNBM99-ClFatB4 or pNBM99-EnClFatB4 express the acyl-[ACP] thioesterase gene (ClFatB4) from Cuphea lanceolata under the control of its own seed specific promoter. Myristic- and palmitic-[ACP] are the substrates of this thioesterase.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record