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Modified Organism
SYN-IR162-4 x SYN-IR6Ø4-5 x MON-ØØØ21-9 - Insect resistant, herbicide tolerant maize
Record information and status
Record ID
110984
Status
Published
Date of creation
2016-10-11 18:00 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-10-11 18:00 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant, herbicide tolerant maize
Transformation event
MIR162 x MIR604 x GA21
Unique identifier
SYN-IR162-4 x SYN-IR6Ø4-5 x MON-ØØØ21-9
Developer(s)
Syngenta France SAS
12, Chemin de l`Hobit,
Saint-Sauveur
France, 31790
Description
The stacked maize line SYN-IR162-4 x SYN-IR6Ø4-5 x MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of mCry3A, PAT, Vip3Aa, EPSPS and PMI genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glyphosate herbicide and allows the LMO to use mannose as a carbon source.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-IR162-4 - Agrisure™ Viptera maize modified to increase insect tolerance
Mannose tolerance Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes
Show detection method(s)
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
pNOV1300, pZM26 and pDPG434
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Metallothionein-like gene promoter
2.56 Kb
 
 
mCry3A
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
transcription start
 
 
Rice actin 1 gene promoter
1.37 Kb
 
 
Rice actin 1, intron
0.00 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase
1.31 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Vegetative insecticidal protein 3Aa20
2.37 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from GA21, vector pDPG434
The GA21 line of maize was genetically engineered, by particle acceleration (biolistic) transformation, to be tolerant of glyphosate-containing herbicides.

DNA insert from MIR162 vector pNOV1300
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests  i it also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

DNA insert from MIR604 vector pZM26
MIR604 is a genetically modified maize developed to confer field protection against corn root worms. The mcry3A gene from Bacillus thuringiensis codes for a  Bt-toxin (Cry3A), which confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Feed

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record