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Modified Organism
Soy modified for the expression of heat-labile enterotoxin, B subunit
Record information and status
Record ID
111063
Status
Published
Date of creation
2016-10-17 19:49 UTC (zuzana.doubkova@mzp.cz)
Date of publication
2016-10-25 18:01 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Soy modified for the expression of heat-labile enterotoxin, B subunit
Transformation event
157
Developer(s)
Dr. Oldrich Navratil
senior scientist
Institute of Experimental Botany, AS CR (IEB)
Prague
Czech Republic
Phone:+ 420 225 106 455
Email:ueb@ueb.cas.cz
Url:Institute of Experimental Botany, AS CR
Description
Soy line No. 157 was modified to express a model immunogen for production in soybean seed with the insertion of the Escherichia coli LTB gene, which codes for the non-toxic beta subunit of the temperature labile enterotoxin. The gene under the control of the soybean seed-specific glycinin promoter and terminator.

In addition, a DNA fragment coding for hygromycin phosphotransferase was also inserted into the modified plant, which confers  resistance to the antibiotic hygromycin, which is used for selection of transformed soy plants.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Point of collection or acquisition of the recipient organism
Variety: 'Jack'
Characteristics of the transformation process
Vector
pGly:ER-LTB
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Glycinin gene promoter
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Heat-labile enterotoxin, B subunit gene
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FLAG Tag
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KDEL ER retention signal
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Glycinin gene terminator
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Ubiquitin-ribosomal protein gene promoter
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Hygromycin B phosphotransferase gene
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Ubiquitin-ribosomal protein gene terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The LTB gene was plant codon optimized and modified with the substitution of the bacterial signal peptide with a 20 aa signal peptide from A. thaliana basic chitinase. Furthermore a 14 aa extension comprising the FLAG epitope and KDEL ER retention signal, and flanking Bsp120 restriction sites were introduced by PCR.

The final sequence encoded a 137 aa protein of 15.5 kDa that yielded a 13.3 kDa LTB-FLAG protein after signal peptide cleavage.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research