Soy modified for the expression of heat-labile enterotoxin, B subunit
Date of creation
2016-10-17 19:49 UTC (firstname.lastname@example.org)
Date of publication
2016-10-25 18:01 UTC (email@example.com)
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Soy modified for the expression of heat-labile enterotoxin, B
Dr. Oldrich Navratil
Institute of Experimental Botany, AS CR (IEB)
Soy line No. 157 was modified to express a model immunogen for
production in soybean seed with the insertion of the
Escherichia coli LTB gene, which codes for the non-toxic
beta subunit of the temperature labile enterotoxin. The gene under
the control of the soybean seed-specific glycinin promoter and
In addition, a DNA fragment coding for hygromycin
phosphotransferase was also inserted into the modified plant, which
confers resistance to the antibiotic hygromycin, which is
used for selection of transformed soy plants.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycinin gene promoter
Heat-labile enterotoxin, B subunit gene
KDEL ER retention signal
Glycinin gene terminator
Ubiquitin-ribosomal protein gene promoter
Hygromycin B phosphotransferase gene
Ubiquitin-ribosomal protein gene terminator
The LTB gene was plant codon optimized and modified with the
substitution of the bacterial signal peptide with a 20 aa signal
peptide from A. thaliana basic chitinase. Furthermore a 14 aa
extension comprising the FLAG epitope and KDEL ER retention signal,
and flanking Bsp120 restriction sites were introduced by PCR.
The final sequence encoded a 137 aa protein of 15.5 kDa that
yielded a 13.3 kDa LTB-FLAG protein after signal peptide cleavage.
- Production of medical or pharmaceutical compounds (human or animal)
- Resistance to antibiotics
- Selectable marker genes and reporter genes