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Record details
Modified Organism
Potato modified for altered phytochrome B expression
LMO Information
Decisions on the LMO
Risk Assessments
Record information and status
Record ID
111078
Status
Published
Date of creation
2016-10-20 13:51 UTC (german_bch@bvl.bund.de)
Date of publication
2016-10-31 16:00 UTC (dina.abdelhakim@cbd.int)
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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the
LMO quick-links
page.
LMO name
Potato modified for altered phytochrome B expression
Transformation event
DARA5 and DARA12
Developer(s)
Record #103100
TU München
Lehrstuhl für Pflanzenzüchtung, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Technische Universität München
Technische Universität München
Alte Akademie 8
Freising, Bayern
Germany, 85354
Phone:
+49 8161 71 -3422
,
+49 8161 71 -3258
Fax:
+49 8161 71-3900
Email:
chris.schoen@wzw.tum.de
,
bork@wzw.tum.de
,
manuela.hochschulte@wzw.tum.de
Url:
TU München, Lehrstuhl Pflanzenzüchtung
,
TU München - Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt
Description
Potato plants are modified to consitutively express the phytochrome B gene from
Arabidopsis thaliana
which codes for a regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses.
As a result of the genetic modification, the anthocyanin and chlorophyll synthesis is enhanced and the photosynthesis rate is increased in the transgenic plants. Furthermore, a number of morphological and physiological changes take place, such as reduced apical dominance, shorter internodes, dwarfism, delayed senescence, thicker stems, starch deposits and increased specific weight in the stems and leaves, smaller leaves, elongation of the palisade parenchyma cells, enhanced root formation, increased number of tubers and increased tuber yield.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Record #12106
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
pMAB316::pTiB6S3-SE
Techniques used for the modification
Agrobacterium-mediated DNA transfer
Genetic elements construct
CaMV 35S promoter
#100287
0.00 Kb
Phytochrome B gene
#111073
0.00 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
Nopaline Synthase Gene Promoter
#100270
0.00 Kb
Neomycin Phosphotransferase II
#15001
0.00 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
Nopaline Synthase Gene Promoter
#100270
0.00 Kb
Nopaline Synthase Gene
#15171
0.00 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
Further details
LMO characteristics
Modified traits
Changes in physiology and/or production
Yield
Growth rate
Photoperiod response
Changes in quality and/or metabolite content
Pigmentation / Coloration
Flavonoids (e.g. anthocyanin)
Resistance to antibiotics
Kanamycin
Streptomycin
Common use(s)
Research
Additional Information
Additional Information
As a major transcription regulator in plants, phytochrome B (PhyB) affects the transcription of a variety of genes. Therefore, while not directly affected by the transformation, the regulation of genes involved in a variety of different physiological and morphological characteristics and metabolic pathways is altered by the elevated PhyB levels in the transgenic plants, such as the phytohormone balance, circadian regulation, regulation of flowering and seed germination, photosynthesis, phototropism and gravitropism, defense responses, the response to cold and the response to visible red and blue light.
Other relevant website address or attached documents
EN
UniProtKB - PhyB - P14713 (PHYB_ARATH)
DE
UniProtKB - PhyB - P14713 (PHYB_ARATH)
Records referencing this document
(
2
)
ID
Description
2
record(s) found
Country's Decision or any other Communication
1 record
Risk Assessment
1 record
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