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Modified Organism
Potato modified for Potato Leaf Roll Virus resistance
Record information and status
Record ID
111084
Status
Published
Date of creation
2016-10-26 12:00 UTC (german_bch@bvl.bund.de)
Date of publication
2016-10-31 19:18 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for Potato Leaf Roll Virus resistance
Transformation event
VR/T18; VR/T21; VR/T23
Developer(s)
MPIPZ
Max Planck Institute for Plant Breeding Research (MPIPZ)
Carl-von-Linné-Weg 10
50829 Köln
Köln
Germany
Phone:+49 221 5062-0
Fax:+49 221 5062-674
Email:prag@mpipz.mpg.de
Url:Homepage of the MPIPZ
Description
Potato plants were modified with the insertion of a consitiutively expressed pr17 gene of the potato leafroll virus, which is presumed to be a transport protein which spreads the viral genome as a ribonucleoprotein complex by the passage through plasmodesmata in the phloem of infected plants.

As a result of the genetic modification, in the event of a PLRV infection, the modified protein could inhibit the formation of the viral transport structures in the genetically modified plants by forming hetero-oligomers with the wild-type transport protein encoded by the viral genome.

This could prevent the long-distance transport of viral RNA in the phloem and consequently the spread and further multiplication of the virus in the plant. Greenhouse experiments revealed that the genetically modified plants were resistant to the potato viruses PLRV, PVX and PVY.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
Derivative of pBIN19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Protein 17 coding sequence
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The PLRV gene coding for the pr17 protein was modified by the addition of a 5' terminal extension by fusion to the multiple cloning site (polylinker) of the vector pBluescript. By targeted mutagenesis, the first two AUG translation start codons of the pr17 gene were changed into ACG codons and an AUG codon was inserted into the polylinker sequence.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record