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Living Modified Organism
(LMO)
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Potato modified with an ethanol-inducible GUS reporter system
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Alc-GUS-22, Alc-GUS-37, Alc-GUS-45
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Organization:Institut für Pflanzengenetik und Kulturpflanzenforschung ()Corrensstrasse 3Gatersleben,
06466 , GermanyPhone: +49 (0)39482 5-0 ,Fax: +49 (0) 39482 5139,Email: info@ipk-gatersleben.de,Website: http://www.ipk-gatersleben.de,
The LM potato was modified to express an ethanol inducible GUS gene from Escherichia coli.
This was carried out by introducing fragments of the ethanol regulon from Aspergillus nidulans into the potato genome in combination with the GUS gene which is under the control of a chimeric promoter consisting of a fragment of the 35S promoter of the Cauliflower Mosaic Virus and a fragment of the promoter of the alcohol dehydrogenase I gene, alcA, from A. nidulans
The fragment of the alcA promoter contains three binding sites for the transcription activator alcR from A. nidulans. which binds to the fragment of alcA of the chimeric promoter in the presence of ethanol thereby inducing transcription.
EN
This was carried out by introducing fragments of the ethanol regulon from Aspergillus nidulans into the potato genome in combination with the GUS gene which is under the control of a chimeric promoter consisting of a fragment of the 35S promoter of the Cauliflower Mosaic Virus and a fragment of the promoter of the alcohol dehydrogenase I gene, alcA, from A. nidulans
The fragment of the alcA promoter contains three binding sites for the transcription activator alcR from A. nidulans. which binds to the fragment of alcA of the chimeric promoter in the presence of ethanol thereby inducing transcription.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
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Derivative of pBIN19
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-111201-1 alcR transactivator gene | Aspergillus nidulans (EMEND)Protein coding sequence | Transcription regulation
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-111202-1 Alcohol dehydrogenase I promoter | Aspergillus nidulans (EMEND)Promoter
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
The cDNA of the alcR transactivator gene is constitutively expressed under the control of the 35S promoter of the CaMV and the terminator sequence of the nopaline synthase gene from Agrobacterium tumefaciens.
The expression of the ß-glucuronidase gene is controlled by a chimeric promoter which consists of a 246 bp fragment of the A. nidulans alcA promoter, containing the three binding sites for the transactivator AlcR, and a fragement of the CaMV-35S promoter (position –31 to +1). The CaMV 35S terminator signal is used for the termination of the transcription.
The neomycin phosphotransferase gene was expressed under the control of the promoter and terminator signals of the nopaline synthase gene from A. tumefaciens and is used as a selection marker.
The introduced nucleic acid is integrated into the genome of the recipient organism.
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The expression of the ß-glucuronidase gene is controlled by a chimeric promoter which consists of a 246 bp fragment of the A. nidulans alcA promoter, containing the three binding sites for the transactivator AlcR, and a fragement of the CaMV-35S promoter (position –31 to +1). The CaMV 35S terminator signal is used for the termination of the transcription.
The neomycin phosphotransferase gene was expressed under the control of the promoter and terminator signals of the nopaline synthase gene from A. tumefaciens and is used as a selection marker.
The introduced nucleic acid is integrated into the genome of the recipient organism.
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- Research
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