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Modified Organism
Potato modified with an ethanol-inducible GUS reporter system
Record information and status
Record ID
111454
Status
Published
Date of creation
2016-11-08 13:43 UTC (german_bch@bvl.bund.de)
Date of publication
2017-01-09 20:28 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified with an ethanol-inducible GUS reporter system
Transformation event
Alc-GUS-22, Alc-GUS-37, Alc-GUS-45
Developer(s)
Institut für Pflanzengenetik und Kulturpflanzenforschung
Corrensstrasse 3 
Gatersleben
Germany, 06466
Phone:+49 (0)39482 5-0
Fax:+49 (0) 39482 5139
Email:info@ipk-gatersleben.de
Url:IPK Gatersleben
Description
The LM potato was modified to express an ethanol inducible GUS gene from Escherichia coli.

This was carried out by introducing fragments of the ethanol regulon from Aspergillus nidulans into the potato genome in combination with the GUS gene which is under the control of a chimeric promoter consisting of a fragment of the 35S promoter of the Cauliflower Mosaic Virus and a fragment of the promoter of the alcohol dehydrogenase I gene, alcA, from A. nidulans

The fragment of the alcA promoter contains three binding sites for the transcription activator alcR from A. nidulans. which binds to the fragment of alcA of the chimeric promoter in the presence of ethanol thereby inducing transcription.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
Derivative of pBIN19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Alcohol dehydrogenase I promoter
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CaMV 35S promoter
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Beta-Glucuronidase coding sequence
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Nopaline Synthase Gene Terminator
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CaMV 35S promoter
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alcR transactivator gene
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Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The cDNA of the alcR transactivator gene is constitutively expressed under the control of the 35S promoter of the CaMV and the terminator sequence of the nopaline synthase gene from Agrobacterium tumefaciens.

The expression of the ß-glucuronidase gene is controlled by a chimeric promoter which consists of a 246 bp fragment of the A. nidulans alcA promoter, containing the three  binding sites for the transactivator AlcR, and a fragement of the CaMV-35S promoter (position -31 to +1). The CaMV 35S terminator signal is used for the termination of the transcription.

The neomycin phosphotransferase gene was expressed under the control of the promoter and terminator signals of the nopaline synthase gene from A. tumefaciens and is used as a selection marker.

The introduced nucleic acid is integrated into the genome of the recipient organism.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record