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Modified Organism
Potato modified for resistance against Potato Virus Y
Record information and status
Record ID
111461
Status
Published
Date of creation
2016-11-21 10:53 UTC (german_bch@bvl.bund.de)
Date of publication
2017-01-10 17:03 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for resistance against Potato Virus Y
Transformation event
GPTV-Kan-NIb-blue Linda NIb36, GPTV-Kan-NIb-blue Linda NIb58, GPTV-Kan-NIb-blue Linda NIb60, GPTV-Kan-NIb-blue Linda NIb80, GPTV-Kan-NIb-blue Linda NIb88, DH59 NIb51, DH59 NIb93, DH59 NIb146, DH59 NIb156
Developer(s)
BAZ
Institut für landwirtschaftliche Kulturen
Bundesanstalt für Züchtungsforschung an Kulturpflanzen (BAZ)
Erwin-Baur-Str. 27
Quedlinburg
Germany, 06484
Phone:+49 (0)3946 47 701
Fax:+49 (0)3946 47 255
Email:zl@jki.bund.de
Url:Julius-Kühn-Institut
Description
The genetically modified potato plants constitutively express a mutated fragment of the NIb gene of the Potato Virus Y, which, in its native form, codes for a RNA-dependent RNA polymerase. The modification is expected to confer resistance to PVY.

To enable the detection the Nib gene product, the modified NIb gene was fused to the enhanced blue fluorescent protein gene from the jellyfish Aequorea victoria as a reporter gene.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Related LMOs
Potato modified for resistance against Potato Virus Y
BAZ Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Viruses - Potato virus Y (PVY) Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pGPTV-Kan-NIb-blue
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
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Nuclear inclusion protein b gene
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Enhanced blue fluorescent protein gene
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CaMV 35S terminator
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CaMV 35S promoter
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Beta-glucuronidase coding sequence
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CaMV 35S terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
A mutated sequence of the NIb replicase of the Potato Virus Y was fused to the enhanced blue fluorescent protein gene. They are expressed under the control of the 35S promoter and termination sequence of the Cauliflower Mosaic Virus.

The mutated sequence has a deletion at the C-terminus (approx. 400 nt) and at the N-terminus (6 nt), and an internal shift in the reading frame affecting 14 amino acids, leading to a non-functional gene product.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Risk Assessment1 record

   
   
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