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Modified Organism
MON-87403-1 - Corn modified for increased ear biomass
Record information and status
Record ID
111522
Status
Published
Date of creation
2017-01-19 20:21 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-01-19 20:21 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Corn modified for increased ear biomass
Transformation event
MON87403
Unique identifier
MON-87403-1
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Corn was modified with the insertion of the ATHB17 gene from Arabidopsis thaliana, which is a transcriptional repressor that has been shown to play an important role in the modulation of plant growth and development. As a result of the modification the resulting truncated form of the ATHB17 gene is unable to function as a transcriptional repressor since it lacks a functional repression domain therefore leading to increased ear biomass at an early reproductive phase compared to conventional control maize.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PV-ZMAP5714
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S Enhancer
0.00 Kb
 
 
Rice actin 1 gene promoter
1.18 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
HB17 transcription factor gene
0.83 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The promoter region of this LMO consists a duplicated enhancer region from the cauliflower mosaic virus 35S promoter combined with the promoter of the act1 gene, that encodes Actin 1, from Oryza sativa.

In this modified organism, maize-specific splicing of the ATHB17 transcript results in a truncated protein, ATHB17Δ113, which is missing the first 113 N-terminal amino acids that are expressed in Arabidopsis thaliana. The ATHB17∆113 protein retains the ability to form homo- and hetero-dimers and bind to target DNA sequences like the full-length protein, however, it is unable to function as a transcriptional repressor because the protein lacks a functional repression domain.

The plasmid backbone also contained cp4 epsps and aadA expression cassettes. The cp4 epsps expression cassette is regulated by the act1 promoter from Oryza sativa, the act1 intron from Oryza sativa, the CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′ untranslated region from Agrobacterium tumefaciens. The aadA expression cassette is regulated by the bacterial promoter, and 3' untranslated region of an aminoglycosidemodifying enzyme, 3''(9)-O-nucleotidyltransferase from the transposon Tn7.

During transformation both the T-DNA and the cp4 epsps expression cassette were inserted into the maize genome. Subsequently, traditional breeding, segregation, selection and screening were used to isolate those plants that contain the ATHB17 expression cassette and not the cp4 epsps and aadA expression cassettes.

Based on sequencing, PCR and bioinformatic analysis, hte modified LMO contains a single copy of the ATHB17 expression cassette that is stably integrated at a single locus.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication2 records
Risk Assessment2 records