Plasmid vector PV-ZMHT507801 contained two separate T-DNAs, each
delineated by Left and Right Border regions. The first T-DNA,
designated as T-DNA I, contained the dmo expression cassette and
the pat expression cassette.
The second T-DNA, designated as T-DNA II, contains the cp4 epsps
expression cassette. The cp4 epsps expression cassette is regulated
by the Ract1 promoter from Oryza sativa, the Ract1 5′ untranslated
leader from Oryza sativa, the Ract1 intron from Oryza sativa, the
CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′
untranslated region from Agrobacterium tumefaciens.
During transformation, both T-DNAs were inserted into the maize
genome. Subsequently, traditional breeding, segregation, selection
and screening were used to isolate those plants that contain the
dmo and pat expression cassettes (T-DNA I) and do not contain the
cp4 epsps expression cassette (T-DNA II).
Molecular characterization of MON 87419 indicated that a single
copy of T-DNA I was integrated into the maize genome at a single
intact locus that includes all expected elements within the insert,
with the exception of incomplete Right and Left Border sequences.
These analyses also showed no PV-ZMHT507801 backbone elements or
T-DNA II sequences were present in the event.
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