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Modified Organism
MON-87419-8 - Dicamba and Glufosinate Tolerant Maize
Record information and status
Record ID
111531
Status
Published
Date of creation
2017-01-20 19:36 UTC (dina.abdelhakim@cbd.int)
Date of last update
2017-07-13 21:06 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-07-13 21:06 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Dicamba and Glufosinate Tolerant Maize
Transformation event
MON87419
Unique identifier
MON-87419-8
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Maize modified with the insertion of transformation cassette containing the Dicamba monooxygenase (DMO) gene and Phosphinothricin N-acetyltransferase (PAT) gene to confer resistance to the herbicides Dicamba and Glufosinate respectively.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PV-ZMHT507801
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Rice actin 1 gene promoter
0.84 Kb
 
 
Rice actin 1, leader sequence
0.08 Kb
 
 
Rice actin 1, intron
0.45 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
PC1SV Promoter
0.43 Kb
 
 
Peroxidase coding sequence
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Chloroplast Transit Peptide 4
0.22 Kb
 
 
Dicamba monooxygenase gene
1.02 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Plasmid vector PV-ZMHT507801 contained two separate T-DNAs, each delineated by Left and Right Border regions. The first T-DNA, designated as T-DNA I, contained the dmo expression cassette and the pat expression cassette.

The second T-DNA, designated as T-DNA II, contains the cp4 epsps expression cassette. The cp4 epsps expression cassette is regulated by the Ract1 promoter from Oryza sativa, the Ract1 5′ untranslated leader from Oryza sativa, the Ract1 intron from Oryza sativa, the CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′ untranslated region from Agrobacterium tumefaciens.

During transformation, both T-DNAs were inserted into the maize genome. Subsequently, traditional breeding, segregation, selection and screening were used to isolate those plants that contain the dmo and pat expression cassettes (T-DNA I) and do not contain the cp4 epsps expression cassette (T-DNA II).

Molecular characterization of MON 87419 indicated that a single copy of T-DNA I was integrated into the maize genome at a single intact locus that includes all expected elements within the insert, with the exception of incomplete Right and Left Border sequences. These analyses also showed no PV-ZMHT507801 backbone elements or T-DNA II sequences were present in the event.
LMO characteristics
Modified traits
  • Resistance to Dicamba
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication2 records
Risk Assessment2 records