The Streptomyces viridochromogenes phosphinothricin
N-acetyltransferase (PAT) gene is under the control of the
Andropogon gerardii ubiquitin (Ubq) gene promoter
and the Oryza sativa alpha-amylase/trypsin inhibitor
terminator. The transcript includes the A. gerardii 5'
untranslated leader sequence and an intron from Ubq before
the PAT.
The Stenotrophomonas maltophilia Dicamba monooxygenase
(DMO) gene is under control of the peanut chlorotic streak
caulimovirus (PC1SV) Full-length transcipt (FLt) promoter and the
Triticum aestivum (wheat) heat shock protein 17
terminator. The transcript produced contains the wheat chlorophyll
a/b-binding 5' untranslated leader sequence (for improved gene
expression), the Oryza sativa Actin 1 untranslated region
(UTR) and intron (for improved gene expression), the targeting and
UTR of Petunia hybrida Chloroplast Transit Peptide 4, and
DMO.
Note:
- Originally, the plasmid vector contained two T-DNA elements that
were inserted during the initial transformation event: one
containing the dmo and pat expression cassettes, and a second
containing a cp4 epsps expression cassette. The cp4 epsps
expression cassette is regulated by the Ract1 promoter from Oryza
sativa, the Ract1 5′ untranslated leader from Oryza sativa, the
Ract1 intron from Oryza sativa, the CTP2 targeting
sequence from Arabidopsis thaliana, and the nos 3′
untranslated region from Agrobacterium tumefaciens.
Subsequent traditional breeding, segregation, selection, and
screening were used to isolate those plants that contain the dmo
and pat expression cassettes (T-DNA I) and do not contain the cp4
epsps expression cassette (T-DNA II).
- Molecular characterization of MON 87419 indicated that a single
copy of T-DNA I was integrated into the maize genome at a single
intact locus that includes all expected elements within the insert,
with the exception of incomplete Right and Left Border sequences.
These analyses also showed no PV-ZMHT507801 backbone elements or
T-DNA II sequences were present in the event.
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