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Modified Organism
Potato modified for increased tolerance to fungal pathogens
Record information and status
Record ID
111620
Status
Published
Date of creation
2017-02-03 12:25 UTC (german_bch@bvl.bund.de)
Date of publication
2017-02-13 16:37 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for increased tolerance to fungal pathogens
Transformation event
Dst9-3, Dst9-6, Dst22-6, Dst23-1, Dst23-15, Dst23-17 and Dst65-24
Developer(s)
MPIPZ
Max Planck Institute for Plant Breeding Research (MPIPZ)
Carl-von-Linné-Weg 10
50829 Köln
Köln
Germany
Phone:+49 221 5062-0
Fax:+49 221 5062-674
Email:prag@mpipz.mpg.de
Url:Homepage of the MPIPZ
Description
Potatoes were modified to express the ribonuclease barnase from Bacillus amyloliquefaciens under the control of the pathogen inducable prp-1 promoter from Solanum tuberosum. The genetically modified plants also constitutively express the barstar gene from Bacillus amyloliquefaciens which is a specific inhibitor protein for barnase.

As a result of the genetic modification the ribonuclease barnase is expressed upon pathogen infection, leading to the cell death of infected cells and restricting the spreading of the fungal pathogen. Since the prp1-1 promoter shows some activity even without pathogen infection, the constitutively expressed inhibitor barstar prevents the cell death of uninfected cells.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
pTCV15 or pTCV17
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Pathogen-defence gene promoter
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Alkaline phosphatase transit peptide
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Barnase
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Nopaline Synthase Gene Terminator
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CaMV 35S promoter
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Barstar
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Transcript 7 gene 3' untranslated region
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Octopine Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The pTCV17 vector was used to develop lines Dst9-3, Dst9-6 and Dst22-6 which contain a 237bp fragment of the prp1-1 promoter.

The pTCV15 vector was used to develop lines Dst23-1, Dst23-15, Dst23-17 and Dst65-24 which contain a 432bp fragment of the prp1-1 promoter.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record