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Modified Organism
Tobacco modified for xylanase production
Record information and status
Record ID
111621
Status
Published
Date of creation
2017-02-06 09:57 UTC (german_bch@bvl.bund.de)
Date of publication
2017-02-13 17:01 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Tobacco modified for xylanase production
Transformation event
XynZ No 34 and XynZ No 46
Developer(s)
Institut für Pflanzengenetik und Kulturpflanzenforschung
Corrensstrasse 3 
Gatersleben
Germany, 06466
Phone:+49 (0)39482 5-0
Fax:+49 (0) 39482 5139
Email:info@ipk-gatersleben.de
Url:IPK Gatersleben
Description
The genetically modified tobacco plants constitutively express a shortened version of the xylanase gene from Clostridium thermocellum that is N-terminally fused to the signal peptide of the proteinase inhibitor II from potato, which leads to the gene product being into the apoplast.

Xylan is a component of the hemicellulose of plant cell walls. It is degraded by xylanases. The transport into the apoplast removes the xylanase from the protease-rich intracellular space, preventing it from being degraded.

Despite the presence of the xylanase, no alteration in plant growth, susceptibility to pathogens and cell wall composition was observed in the transgenic tobacco plants.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Nicotiana tabacum - Tobacco, TOBAC
Characteristics of the transformation process
Vector
Derivative of pBIN19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Proteinase inhibitor II transit peptide
0.00 Kb
 
 
Xylanase Z gene
0.00 Kb
 
 
Octopine Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
LMO characteristics
Modified traits
  • Use in industrial applications
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record