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Modified Organism
Sinorhizobium meliloti modified for the expression of a reporter gene and reduced growth
Record information and status
Record ID
111660
Status
Published
Date of creation
2017-02-22 12:51 UTC (german_bch@bvl.bund.de)
Date of publication
2017-02-22 17:02 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Sinorhizobium meliloti modified for the expression of a reporter gene and reduced growth
Transformation event
L1
Developer(s)
Uni Bielefeld
Universität Bielefeld
Universitätsstraße 25
33615 Bielefeld
Bielefeld
Germany, 33615
Phone:+49 521 106-00
Fax:+49 521 106-5844
Email:post@uni-bielefeld.de
Url:Bielefeld University
Description
The genetically modified sinorhizobia constitutively express the luciferase gene from Photinus pyralis coding for the luciferin-4-monooxygenase which catalyses a light-producing reaction and is therefore used as a reporter gene.

The luciferase expression cassette is integrated into the recA gene of the genome of S. meliloti which plays a central role in natural recombination processes, DNA repair and the control of gene expression after DNA damage. Insertion of the luciferase expression cassette, disrupts the functionality of the endogenous recA gene product.

As a result of the genetic modification S. meliloti has a prolonged generation time and a growth disadvantage.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Sinorhizobium meliloti - RHIML
Point of collection or acquisition of the recipient organism
The S. meliloti strain Rm2011 was used for the transformation.
Related LMOs
Sinorhizobium meliloti modified for the expression of a reporter gene
Uni Bielefeld Selectable marker genes and reporter genes
Characteristics of the transformation process
Techniques used for the modification
  • Direct DNA transfer
Genetic elements construct
 
Neomycin phosphotransferase II promoter
0.00 Kb
 
 
Firefly Luciferase
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The luciferase expression cassette was inserted into the coding sequence of the recA gene by in vivo recombination, leading to a non-functional recA gene product.

The construct did not contain its own termination sequence. It was site-specifically integrated into the S. meliloti recA Gene. The insertion site is located upstream of the endogenous recA termination region, therefore, transcription termination would be controlled by that sequence.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Other gene(s) whose expression was affected by the transformation
Recombinase A gene - Sinorhizobium meliloti - RHIML
Changes in physiology and/or production - Growth rate
How the expression of the gene(s) was affected
The insertion of the luc expression cassette into the coding sequence of the recA gene leads to a loss of function of the recA gene product.
Common use(s)
  • Research
Additional Information
Additional Information
S. meliloti needs a host plant to grow and proliferate properly. Here, the symbiotic interaction partner alfalfa, Medicago sativa, is used as a host plant.

Records referencing this document (1)
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1record(s) found
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