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Modified Organism
BCS-GHØØ2-5 × MON-15985-7 - Herbicide-tolerant, insect-resistant cotton
Record information and status
Record ID
111987
Status
Published
Date of creation
2017-06-05 14:53 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-06-05 14:53 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant, insect-resistant cotton
Transformation event
GHB614 x MON15985
Unique identifier
BCS-GHØØ2-5 × MON-15985-7
Developer(s)
Description
The stacked cotton line  was generated through the traditional cross breeding of each of parental organisms to express the cry1A(c), cry2A(b), and 2mepsps genes. The expression of these genes confers resistance against lepidoptera pests and tolerance to glyphosate herbicides.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
MON-15985-7 - Bollgard II™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
BCS-GHØØ2-5 - GlyTol™ Cotton GHB614
Bayer CropScience Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GHBK11, PV-GHBK04 and pTEM2
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Beta-Glucuronidase coding sequence
1.81 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Cry1Ac
0.35 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.63 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
HSP 70 5' untranslated leader sequence
0.10 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Histone H4 gene Promoter
1.01 Kb
 
 
Histone H3 Gene II intron 1
0.52 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
Double Mutant 5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Histone H4 gene 3' UTR
0.74 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from 15985 vector PV-GHBK11
The T-DNA from this vector contributed the coding sequences of the cry2A(b) and uidA genes. These allow for the expression of the insecticidal cry2A(b) crystal protein which protects against lepidoptera pests and the Beta-Glucuronidase selectable marker.

DNA insert from 15985 vector PV-GHBK04
Originally integrated into the MON531 line and persisted through to the 15985 line. The T-DNA from this vector contains the coding sequence of the cry1A(c)  gene and the nptII resistance gene. The aadA gene was also integrated into the host genome however it is not expressed.

DNA insert from GHB614 vector pTEM2
Cotton event GHB614 contains a stably integrated 2mepsps gene. This modification confers to the protein a decreased binding affinity for the herbicide glyphosate, allowing it to maintain sufficient enzymatic activity and thus tolerance in its presence.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
Additional Information
Other relevant website address or attached documents