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Modified Organism
SYN-E3272-5 x SYN-IR6Ø4-5 - Modified thermostable alpha-amylase, insect-resistant, herbicide-tolerant maize
Record information and status
Record ID
111995
Status
Published
Date of creation
2017-06-07 14:51 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-06-07 14:51 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Modified thermostable alpha-amylase, insect-resistant, herbicide-tolerant maize
Transformation event
3272 x MIR604
Unique identifier
SYN-E3272-5 x SYN-IR6Ø4-5
Developer(s)
Syngenta Seeds GmbH
Syngenta Seeds GmbH
Zum Knipkenbach 20
Bad Salzuflen
Germany, 32107
Phone:+49 52 22 5308-0
Fax:+49 52 22 5308-12
Email:info.seeds@syngenta.com
Url:Syngenta Seeds Germany
Description
The stacked maize line SYN-E3272-5 and SYN-IR6Ø4-5 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of amy797E alpha amylase, PMI and  mCry3A genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera
as well as the synthesis of thermostable alpha-amylase.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes
Show detection method(s)
SYN-E3272-5 - Enogen™ Maize
Mannose tolerance Selectable marker genes and reporter genes Thermostable alpha-amylase Use in industrial applications - Biofuel production
Show detection method(s)
Characteristics of the transformation process
Vector
pNOV7013 and pZM26
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Metallothionein-like gene promoter
2.56 Kb
 
 
mCry3A
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
27kD gamma-zein Promoter
0.68 Kb
 
 
amy797E alpha amylase
1.38 Kb
 
 
SEKDEL ER retention signal
0.01 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from 3272 vector pNOV7013
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.

DNA insert from MIR604 vector pZM26
MIR604 is a genetically modified to confer field protection against corn root worms. The cry3A gene codes for a Bt-toxin (Cry3A), which confers resistance to coleopteran species. Expression of the pmi gene allows the plant to use mannose as a carbon source through production of the PMI protein, and is used as a selectable marker.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Biofuel