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Modified Organism
DAS-Ø15Ø7-1 x DAS-59122-7 x MON-ØØ81Ø-6 - Insect resistant, herbicide tolerant maize
Record information and status
Record ID
112033
Status
Published
Date of creation
2017-06-15 14:07 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-06-15 14:07 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant, herbicide tolerant maize
Transformation event
TC1507 x 59122 x MON810
Unique identifier
DAS-Ø15Ø7-1 x DAS-59122-7 x MON-ØØ81Ø-6
Developer(s)
Dupont Poineer
Chestnut Run Plaza 720/1S5
974 Centre Road
Wilmington, Delaware
United States of America, 19805
Description
The stacked maize line DAS-Ø15Ø7-1 x DAS-59122-7 x MON-ØØ81Ø-6 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of Cry1F, PAT, Cry34Ab1, Cry35Ab1 and Cry1Ab genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PHI8999A, PHP17662, PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
1.99 Kb
 
 
Cry34Ab1
0.37 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Peroxidase gene promoter
1.30 Kb
 
 
Cry35Ab1
1.15 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome. MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from TC1507 vector PHI8999A
The TC1507 line contained the Cry1F and PAT coding sequences and regulatory elements. The Cry1F gene produces insecticidal crystal protein that confers resistance against lepidopteran pests and the PAT gene produces Phosphinothricin N-acetyltransferase which confers tolerance to the glufosinate herbicide.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents