Insect resistant, herbicide tolerant maize

A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of SYN-BTØ11-1 x DAS-59122-7 x DAS-Ø15Ø7-1 x MON-ØØØ21-9.  Insect resistance is conferred by the cry1A(b), cryIF, cry34Ab1, and cry35Ab1 genes from the common soil bacterium Bacillus thuringiensis, and glufosinate and glyphosate herbicide tolerance through the pat and EPSPS genes respectively.

pZO1502, pDPG434, PHP17662 and PHI8999A

DNA insert from Bt 11 vector pZO1502
Contributed the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene  to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from GA21 vector pDPG434
The 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) gene from maize was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate. The resulting gene was used to develop GA21

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from TC1507 vector PHI8999A
TC1507 modified with the insertion of the Cry1F geneto confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

For additional information on this LMO, please refer to the records of the parental LMOs.