| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
Herbicide tolerant Maize
Record information and status
Record ID
112264
Status
Published
Date of creation
2017-07-17 15:46 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-07-17 15:46 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant Maize
Transformation event
HCEM485
Developer(s)
Stine Seed Farm, Inc.
Adel
United States of America
Description
HCEM485 maize line was modified for glyphosate herbicide tolerance by inserting a 6.0 kb maize genomic fragment containing a modified, glyphosate tolerant form of the endogenous maize EPSPS encoding gene.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Parent line: Stine 963
Related LMOs
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
pHCEM
Techniques used for the modification
  • Direct DNA transfer
Genetic elements construct
 
EPSPS gene promoter
0.00 Kb
 
 
Double Mutant 5-enolpyruvylshikimate-3-phosphate synthase
0.00 Kb
 
 
EPSPS gene terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The maize EPSPS coding sequence was specifically modified by site-directed mutagenesis to introduce two single-nucleotide substitutions. These two point mutations resulted in a codon change from threonine to isoleucine  at position 102 (relative to the amino acid sequence of the native maize EPSPS enzyme) and
a proline to serine change at position 106.

Molecular characterization of the insertion suggests that 4 complete copies of the fragment are inserted at a single location.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents