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Modified Organism
DAS-81419-2 x DAS-444Ø6-6 - Conkesta Enlist E3™ Soybean
Record information and status
Record ID
112356
Status
Published
Date of creation
2017-08-17 14:29 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-08-17 14:29 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Conkesta Enlist E3™ Soybean
Transformation event
DAS-81419-2 x DAS-44406-6
Unique identifier
DAS-81419-2 x DAS-444Ø6-6
Developer(s)
Description
The stacked soybean line Conkesta Enlist E3™ was created through the conventional cross-breeding of the LM cotton lines DAS-44406-6 and DAS-81419-2 to express aad-12, 2mepsps and pat genes to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D), glyphosate and glufosinate respectively. The LM plant also expresses Cry1F and Cry1Ac each of which expresses insecticidal crystal proteins thus conferring resistance against lepidoptera.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
DAS-444Ø6-6 - Enlist E3™ Soybean
Resistance to herbicides - Glufosinate, Glyphosate
DAS-81419-2 - Conkesta™ Soybean
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Characteristics of the transformation process
Vector
pDAB8264 and pDAB9582
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
RB7 matrix attachment region
1.17 Kb
 
 
Histone H4 gene 3' UTR
0.66 Kb
 
 
Double Mutant 5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
Histone H3 Gene II intron 1
0.00 Kb
 
 
Histone H4 gene Promoter
1.43 Kb
 
 
Polyubiquitin10 gene promoter
1.32 Kb
 
 
Aryloxyalkanoate dioxygenase gene
0.88 Kb
 
 
ORF23 3' Untranslated region
0.46 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
ORF1 3' Untranslated region
0.17 Kb
 
 
Polyubiquitin10 gene promoter
1.32 Kb
 
 
Cry1F
3.45 Kb
 
 
ORF23 3' Untranslated region
0.46 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Cry1Ac
3.47 Kb
 
 
ORF23 3' Untranslated region
0.48 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from DAS-44406-6 vector pDAB8264
DAS-44406-6 soybean was modified with the insertion of a gene encoding aryloxyalkanoate dioxygenase and phosphinothricin N-acetyltransferase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate respectively. Further more a gene encoding a modified version of the EPSPS gene was inserted to confer tolerance to glyphosate.

DNA insert from DAS-81419-2 vector pDAB9582
Soy line DAS-81419-2 was transformed with the insertion of Cry1F and Cry1Ac to confer resistance to lepidopteran insects as well as the insertion of the pat gene which results in the synthesis of phosphinothricin N-acetyltransferase thus coffering tolerance to glufosinate herbicides.

For additional information on this LMO, please refer to the records of the parental LMOs
LMO characteristics
Modified traits
  • Tolerance to 2,4-Dichlorophenoxyacetic acid
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication2 records
Risk Assessment2 records