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Modified Organism
DAS-59122-7 x MON-88Ø17-3 x DAS-4Ø278-9 - Insect resistant, herbicide tolerant maize
Record information and status
Record ID
112359
Status
Published
Date of creation
2017-08-18 16:43 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-08-18 16:44 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant, herbicide tolerant maize
Transformation event
59122 x MON88017 x DAS40278
Unique identifier
DAS-59122-7 x MON-88Ø17-3 x DAS-4Ø278-9
Developer(s)
Dow AgroSciences GmbH
Dow AgroSciences GmbH
Truderinger Straße 15
München, Bayern
Germany, 81677
Phone:+ 49 89 - 4 55 33 - 0
Fax:+ 49 89 - 4 55 33 - 111
Email:DowAgroSciencesD@dow.com
Url:http://www.dowagro.com/de
Description
The stacked maize line DAS-59122-7 x MON-88Ø17-3 x DAS-4Ø278-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of aryloxyalkanoate dioxygenase, PAT, EPSPS, Cry3Bb1, Cry34Ab1 and Cry35Ab1 genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors, glufosinate herbicide and glyphosate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glyphosate
Show detection method(s)
DAS-4Ø278-9 - Enlist™ Maize
Dow AgroSciences GmbH Resistance to herbicides Tolerance to 2,4-Dichlorophenoxyacetic acid Tolerance to aryloxyphenoxypropionate
Show detection method(s)
Characteristics of the transformation process
Vector
pDAS1740, PHP17662 and PV-ZMIR39
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
RB7 matrix attachment region
1.17 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Aryloxyalkanoate dioxygenase gene
0.89 Kb
 
 
Per5 3' Untranslated Region
0.37 Kb
 
 
RB7 matrix attachment region
1.17 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.07 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Cry3Bb1
1.96 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.23 Kb
 
 
Rice actin 1 gene promoter
0.93 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Cry34Ab1
0.37 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Peroxidase gene promoter
1.30 Kb
 
 
Cry35Ab1
1.15 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from DAS40278 vector pDAS1740
DAS-40278-9 corn was modified with the insertion of a plant optimised coding sequence for aryloxyalkanoate dioxygenase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors.

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from MON88017 vector PV-ZMIR39
MON88017 expresses the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein to control infestation with corn root worm, and the cp4 epsps gene.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Tolerance to 2,4-Dichlorophenoxyacetic acid
  • Tolerance to aryloxyphenoxypropionate
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents