Record details

Living Modified Organism identity

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LMO name

Sugarcane modified for insect resistance

Transformation event

CTB141175/01

Unique identifier

CTC-14117-4

Developer(s)

Record #112453

Dr Wladecir Salles Oliveira

Chairman of the Internal Biosafety Committee

CTC – Centro de Tecnologia Canavieira (CTC)

Fazenda Santo Antônio s/n, Caixa Postal 162

Piracicaba, Sao Paulo

Brazil, 13400-970

Phone:	+55 (11) 3429-8111
Fax:	+55 (11) 3429-8111
Email:	Wladecir.oliveira@ctc.com.br
Url:	CTC

Description

Sugarcane was modified with the insertion of gene cry1Ab to confer resistance to sugarcane borer, Diatraea saccharalis. The gene encoding neomycin phosphotransferase II, which confers resistance to aminoglycoside antibiotics such as kanamycin, was also integrated into the sugarcane as a selection marker.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #100332

Saccharum sp. - Sugar cane

Point of collection or acquisition of the recipient organism

Cultivar: CTC20

Related LMOs

Record #100333

Sugar cane resistant to insects

Characteristics of the transformation process

Vector

pGH-CTC2.nptII

Techniques used for the modification

Biolistic / Particle gun

Genetic elements construct

Phosphoenolpyruvate Carboxylase gene promoter

#101404

2.30 Kb

Cry1Ab

#14985

1.90 Kb

Nopaline Synthase Gene Terminator

#100269

0.30 Kb

Ubiquitin gene promoter

#100362

2.00 Kb

Neomycin Phosphotransferase II

#15001

0.80 Kb

Nopaline Synthase Gene Terminator

#100269

0.30 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

Sugarcane is vegetatively propagated for commercial production. There is minimal likelihood of transgene introgression via pollination.

CTB141175/01-A is a cultivar specific event. Southern blot analysis and sequence analysis indicated that multiple expression cassettes were integrated into the CTB141175/01-A event.

Southern blot analysis up to 6 generations indicated that all insertions were stable across several vegetative propagation cycles.

Western blot results confirmed the presence and integrity of Cry1Ab and NPTII proteins and absence of any other non-intended proteins.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Lepidoptera (butterflies and moths)

Common use(s)

Food
Feed

Additional Information

Other relevant website address or attached documents

CTB141175/01-A - ISAAA