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Modified Organism
CTC-14117-4 - Sugarcane modified for insect resistance
Record information and status
Record ID
112454
Status
Published
Date of creation
2017-08-29 18:35 UTC (gutemberg.sousa@mctic.gov.br)
Date of last update
2017-08-30 18:59 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-08-30 18:59 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Sugarcane modified for insect resistance
Transformation event
CTB141175/01
Unique identifier
CTC-14117-4
Developer(s)
Dr Wladecir Salles Oliveira
Chairman of the Internal Biosafety Committee
CTC – Centro de Tecnologia Canavieira (CTC)
Fazenda Santo Antônio s/n, Caixa Postal 162
Piracicaba, Sao Paulo
Brazil, 13400-970
Phone:+55 (11) 3429-8111
Fax:+55 (11) 3429-8111
Email:Wladecir.oliveira@ctc.com.br
Url:CTC
Description
Sugarcane was modified with the insertion of gene cry1Ab to confer resistance to sugarcane borer, Diatraea saccharalis. The gene encoding neomycin phosphotransferase II, which confers resistance to aminoglycoside antibiotics such as kanamycin, was also integrated into the sugarcane as a selection marker.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Saccharum sp. - Sugar cane
Point of collection or acquisition of the recipient organism
Cultivar: CTC20
Related LMOs
Sugar cane resistant to insects
Characteristics of the transformation process
Vector
pGH-CTC2.nptII
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Phosphoenolpyruvate Carboxylase gene promoter
2.30 Kb
 
 
Cry1Ab
1.90 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
Ubiquitin gene promoter
2.00 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Sugarcane is vegetatively propagated for commercial production. There is minimal likelihood of  transgene introgression via pollination.

CTB141175/01-A is a cultivar specific event. Southern blot analysis and sequence analysis indicated that multiple expression cassettes were integrated into the CTB141175/01-A event.

Southern blot analysis up to 6 generations indicated that all insertions were stable across several vegetative propagation cycles.

Western blot results confirmed the presence and integrity of Cry1Ab and NPTII proteins and absence of any other non-intended proteins.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents