Record details

Living Modified Organism identity

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LMO name

Insect resistant, herbicide tolerant maize

Transformation event

TC1507 x DAS40278

Unique identifier

DAS-Ø15Ø7-1 x DAS-4Ø278-9

Developer(s)

Record #14939

Dow AgroSciences

Url:	Dow AgroSciences Homepage

Description

The stacked maize line DAS-Ø15Ø7-1 x DAS-4Ø278-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of aryloxyalkanoate dioxygenase, pat and Cry1F genes. The expression of these genes are expected to confer resistance to Lepidoptera, and tolerant to 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors and the herbicide glufosinate.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #14841

DAS-Ø15Ø7-1 - Herculex™ I maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate

Show detection method(s)

Record #104814

DAS-4Ø278-9 - Enlist™ Maize

Dow AgroSciences GmbH Resistance to herbicides Tolerance to 2,4-Dichlorophenoxyacetic acid Tolerance to aryloxyphenoxypropionate

Show detection method(s)

Characteristics of the transformation process

Vector

pDAS1740 and PHI8999A

Techniques used for the modification

Cross breeding

Genetic elements construct

RB7 matrix attachment region

#104795

1.17 Kb

Ubiquitin gene promoter

#100362

1.99 Kb

Aryloxyalkanoate dioxygenase gene

#104812

0.89 Kb

Per5 3' Untranslated Region

#104813

0.37 Kb

RB7 matrix attachment region

#104795

1.17 Kb

Ubiquitin gene promoter

#100362

1.98 Kb

Cry1F

#14987

1.82 Kb

ORF25 PolyA Terminator sequence

#100363

0.72 Kb

CaMV 35S promoter

#100287

0.55 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

CaMV 35S terminator

#100290

0.20 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from TC1507 vector PHI8999A
The TC1507 line contained the Cry1F and PAT coding sequences and regulatory elements. The Cry1F gene produces insecticidal crystal protein that confers resistance against lepidopteran pests and the PAT gene produces Phosphinothricin N-acetyltransferase which confers tolerance to the glufosinate herbicide.

DNA insert from DAS40278 vector pDAS1740
DAS-40278-9 corn was modified with the insertion of a plant optimised coding sequence for aryloxyalkanoate dioxygenase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors.

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Lepidoptera (butterflies and moths)
Resistance to herbicides
- Glufosinate
Tolerance to 2,4-Dichlorophenoxyacetic acid
Tolerance to aryloxyphenoxypropionate

Common use(s)

Food
Feed

Detection method(s)

External link(s)

DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

DAS-4Ø278-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

Additional Information

Other relevant website address or attached documents

DAS-Ø15Ø7-1 x DAS-4Ø278-9 - ISAAA