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Modified Organism
DAS-Ø15Ø7-1 x DAS-59122-7 x DAS-4Ø278-9 - Insect resistant, herbicide tolerant maize
Record information and status
Record ID
112458
Status
Published
Date of creation
2017-09-01 15:20 UTC (dina.abdelhakim@cbd.int)
Date of last update
2017-09-01 17:23 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-09-01 17:23 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant, herbicide tolerant maize
Transformation event
TC1507 x 59122 x DAS40278
Unique identifier
DAS-Ø15Ø7-1 x DAS-59122-7 x DAS-4Ø278-9
Developer(s)
Description
The stacked maize line DAS-Ø15Ø7-1 x DAS-59122-7 x DAS-4Ø278-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of aryloxyalkanoate dioxygenase, Cry1F, PAT, Cry34Ab1 and Cry35Ab1 genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors and glufosinate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-4Ø278-9 - Enlist™ Maize
Dow AgroSciences GmbH Resistance to herbicides Tolerance to 2,4-Dichlorophenoxyacetic acid Tolerance to aryloxyphenoxypropionate
Show detection method(s)
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pDAS1740, PHP17662, PV-ZMIR39, PHI8999A and PV-ZMIR245
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Cry34Ab1
0.37 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Peroxidase gene promoter
1.30 Kb
 
 
Cry35Ab1
1.15 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
RB7 matrix attachment region
1.17 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Aryloxyalkanoate dioxygenase gene
0.89 Kb
 
 
Per5 3' Untranslated Region
0.37 Kb
 
 
RB7 matrix attachment region
1.17 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from TC1507 vector PHI8999A
The TC1507 line contained the Cry1F and PAT coding sequences and regulatory elements. The Cry1F gene produces insecticidal crystal protein that confers resistance against lepidopteran pests and the PAT gene produces Phosphinothricin N-acetyltransferase which confers tolerance to the glufosinate herbicide.

DNA insert from DAS40278 vector pDAS1740
DAS-40278-9 corn was modified with the insertion of a plant optimised coding sequence for aryloxyalkanoate dioxygenase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors.

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Tolerance to aryloxyphenoxypropionate
  • Tolerance to 2,4-Dichlorophenoxyacetic acid
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents