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Modified Organism
Cassava modified for reduced starch metabolism
Record information and status
Record ID
112609
Status
Published
Date of creation
2017-10-05 15:04 UTC (bchnigeria@gmail.com)
Date of publication
2017-10-17 20:05 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cassava modified for reduced starch metabolism
Transformation event
AMY3 RNAi
Developer(s)
Livia Stavolone
Research
International Institute of Tropical Agriculture (IITA)
PMB 5320 Oyo Road 200001
Ibadan, Oyo
Nigeria
Phone:+234 700800IITA
Fax:+442087113786
Email:I.stavolone@cgiar.org
Description
Cassava was genetically modified for reduced starch metabolism in the tuber after pruning, prior to crop harvest.

The modification was carried out through the insertion of an RNAi construct that was designed to suppress endogenous expression of each of alpha-amylase 3 which is involved in is involved in the release of branched malto‐olicosaccharides from the starch granule.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
Point of collection or acquisition of the recipient organism
Cultivar 60444
Characteristics of the transformation process
Vector
pCAMBIA1301_patatin AMY3 RNAi
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
B33 gene promotor
0.00 Kb
 
 
Alpha-amylase 3 gene
0.00 Kb
 
 
M27939 Intron Sequence
0.00 Kb
 
 
Alpha-amylase 3 gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Base pairs 57‐165 of the synthetic plant intron sequence M27939 are integrated into the genetic construct.

The α-amylase sequence consist of the first 210 base pairs of the coding sequence.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record