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Modified Organism
DAS-Ø15Ø7-1 x MON-ØØ81Ø-6 x SYN-IR6Ø4-5 - Insect resistant herbicide tolerant maize
Record information and status
Record ID
112717
Status
Published
Date of creation
2017-11-13 20:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-11-13 20:40 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant herbicide tolerant maize
Transformation event
TC1507 x MON810 x MIR604
Unique identifier
DAS-Ø15Ø7-1 x MON-ØØ81Ø-6 x SYN-IR6Ø4-5
Developer(s)
Dupont Poineer
Chestnut Run Plaza 720/1S5
974 Centre Road
Wilmington, Delaware
United States of America, 19805
Description
The stacked maize line TC1507×MON810×MIR604 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of cry1F, pat, cry1Ab, and cry3Aa2 genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
PHI8999A, PV-ZMBK07, and pZM26
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Metallothionein-like gene promoter
2.56 Kb
 
 
mCry3A
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Legumin B4 gene promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from TC1507 vector PHI8999A
TC1507 modified with the insertion of the Cry1F gene to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

DNA insert from MON810 vector PV-ZMBK07
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene which confers resistance against lepidoptera

DNA insert from MIR604 vector pZM26
Cry3A gene was modified for enhanced expression in maize and such that the amino acid sequence of the synthetic version of Cry3A is the same as the native protein, except for the modified serine-protease recognition site. The pmi gene encodes the enzyme phosphomannose isomerase (PMI) that allows the plants to utilise mannose as a carbon source and is used as a selectable marker.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents