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Modified Organism
SPS-ØØX17-5 - Innate® Acclimate Ranger Russet Potato
Record information and status
Record ID
112929
Status
Published
Date of creation
2017-12-22 03:33 UTC (tim.strabala@epa.govt.nz)
Date of publication
2018-01-02 18:07 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Innate® Acclimate Ranger Russet Potato
Transformation event
X17
Unique identifier
SPS-ØØX17-5
Developer(s)
SPS International
J.R. Simplot Company
5369 West Irving Street 
Boise, Idaho
United States of America, 83706
Phone:+1 (208) 780-6066
Email:simplot@simplot.com
Description
Simplot Innate® Acclimate potato is was developed through the retransformation of the SPS-ØØF10-7 Innate™ Ranger Russet Potato line. The resulting organism silences genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes. It also expresses the late blight resistance gene which confers resistance to late blight disease.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
SPS-ØØF10-7 - Innate™ Ranger Russet Potato
Changes in quality and/or metabolite content - Pigmentation / Coloration, Protein and amino acids
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Var. Ranger Russet
Characteristics of the transformation process
Vector
pSIM1678 and pSIM1278
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Vacuolar invertase gene
0.68 Kb
 
 
Vacuolar invertase gene
0.50 Kb
 
 
Granule bound starch synthase gene promoter
0.92 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.23 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.51 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
 
Phytophthora infestans Resistance gene 1 Promoter
0.71 Kb
 
 
Phytophthora infestans Resistance gene 1
2.68 Kb
 
 
Phytophthora infestans Resistance gene 1 terminator
0.93 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from vector pSIM1278
This DNA insert comprises two expression cassettes that were inserted into the pSIM1278 transformation vector.

The first cassette comprises fragments of both the asparagine synthetase-1 gene (Asn1) and the polyphenol oxidase-5 gene (Ppo5), arranged as inverted repeats between the Agp promoter of the ADP  glucose pyrophosphorylase gene (Agp) and the Gbss promoter of the granule-bound starch synthase gene (Gbss) and results in silencing of both the Ppo5 and Asn1 genes.

The second cassette is comprised of fragments of the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL), operably linked to the same Agp and Gbss promoters as the first cassette. The function of the second cassette is to silence the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL).

Molecular analyses indicated that the transformed organism contains a single nearly complete copy of the DNA insert at a single locus and no vector backbone in the LMO. Alignment of the integration site sequences to the MSU potato reference genome indicates the likely integration site is on chromosome 8 for pSIM1278. There are no ORFs covering the left junction associated with the pSIM1278 insert in X17. A single ORF was identified spanning the right junction. It is almost completely native potato sequence.

DNA insert from vector pSIM1678
This DNA insert comprises two expression cassettes that were inserted into the pSIM1678 transformation vector.

The first cassette comprises of the Rpi-vnt1 expression cassette, which consists of the VNT1 protein
coding region regulated by the native Rpi-vnt1 promoter and terminator sequences. VNT1 is an R-protein from the wild Solanum species, S. venturii, which functions in the recognition of P. infestans and elicits a hypersensitive response in potato, conferring resistance to late blight disease.

The second insert consists of a down-regulation cassette for the plant vacuolar invertase gene, VInv, consisting of sequence from the potato VInv gene arranged as an inverted repeat and flanked by opposing plant promoters, pGbss and pAgp. Vacuolar invertase converts sucrose into glucose and fructose. Its down regulation prevents excess darkening during frying and contributes to low acrylamide potential

Molecular analyses indicated that the transformed organism contains a single nearly complete copy of the DNA insert at a single locus and no vector backbone in the LMO. Alignment of the integration site sequences to the MSU potato reference genome indicates the likely integration site is on chromosome 5 for pSIM1678. There are no ORFs covering the left junction associated with the pSIM1678 insert in X17. A single ORF was identified spanning the right junction. It is almost completely native potato sequence.

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None of the X17 junction ORFs were identified as homologs of known toxins or allergens. Each X17 insert is shown to be stable during vegetative propagation.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record