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Modified Organism
SYN-BTØ11-1 x SYN-IR162-4 x MON-89Ø34-3 - Maize modified for insect resistance and herbicide tolerance
Record information and status
Record ID
112994
Status
Published
Date of creation
2018-01-10 17:13 UTC (dina.abdelhakim@cbd.int)
Date of publication
2018-01-10 17:13 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize modified for insect resistance and herbicide tolerance
Transformation event
Bt11 x MIR162 x MON89034
Unique identifier
SYN-BTØ11-1 x SYN-IR162-4 x MON-89Ø34-3
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The stacked maize line SYN-BTØ11-1 x SYN-IR162-4 x MON-89Ø34-3 was obtained through the traditional cross breeding of each of the parental organisms. The line inherited genes that express cry1Ab. cry1A.105, cry2Ab2, vip3Aa19, PAT and PMI. The expression of these genes confer glufosinate herbicide tolerance as well as resistance to Lepidoptera and Coleoptera.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
SYN-IR162-4 - Agrisure™ Viptera maize
Mannose tolerance Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
MON-89Ø34-3 - YieldGard™ VT Pro™
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMIR245, pNOV1300, and pZO1502
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
1.99 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Vegetative insecticidal protein 3Aa20
2.37 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
FMV 35S promoter
0.56 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Transit peptide and first intron of Rubisco SSU
0.40 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV Enhanced 35S promoter
0.30 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry1A.105
3.53 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
 
CaMV 35S promoter
0.51 Kb
 
 
Alcohol Dehydrogenase 1, intron 6
0.47 Kb
 
 
Cry1Ab
1.85 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.42 Kb
 
 
Alcohol Dehydrogenase 1, intron 6
0.18 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON89034 vector PV-ZMIR245
Maize line MON89034 expresses two Bt-toxins encoded by the genes cry1A.105 and cry2Ab2 from Bacillus thuringiensis that confer resistance against certain lepidopteran pests.

DNA insert from MIR162, vector pNOV1300:
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests  including: fall armyworm, armyworm , beet armyworm , corn earworm , black cutworm , and western bean cutworm. Event MIR162 maize also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

DNA insert from Bt 11 vector pZO1502
Maize produced by inserting the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (6)
IDDescription
6record(s) found
Country's Decision or any other Communication3 records
Risk Assessment3 records

   
   
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