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Cotton (Gossypium hirsutum) modified with resistance genes for the
herbicides isoxaflutole and glyphosate via Agrobacterium-mediated
transformation using the vector pTSIH09 containing the
hppdPfW336-1Pa and 2mepsps expression cassettes.
(i) The double mutant 5-enol pyruvylshikimate-3-phosphate synthase
that encodes for the 2mEPSPS protein. The 2mepsps coding sequence
developed by introducing two point mutations to the wild-type epsps
gene cloned from
maize (Zea mays).
(ii) The hppdPf W336 gene encodes for the HPPD W336 protein. The
coding sequence was developed by introducing a single point
mutation to the wild type
hppd gene derived from Pseudomonas fluorescens. Expression of the
protein confers tolerance to isoxaflutole herbicides.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Histone H4 gene Promoter - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
5-enolpyruvylshikimate-3-phosphate synthase - Zea mays - Maize, Corn, MAIZE
Resistance to herbicides - Glyphosate
Histone H3 Gene II intron 1 - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Histone H4 gene 3' UTR - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
CsVMV promoter - Cassava vein mosaic virus - Cassava vein mosaic virus, CVMV, CsVMV
4-Hydroxyphenylpyruvate dioxygenase gene - Pseudomonas fluorescens - PSEFL
Resistance to herbicides
In the 2mepsps gene expression cassettes, the 2mepsps gene coding
sequence is under
the control of the H4 promoter of Arabidopsis thaliana (Ph4A748;
Chabouté et al., 1987),
followed by the first intron of gene II of the histone H3.III
variant of Arabidopsis thaliana
(Chaubet et al., 1992) and by the optimized transit peptide as
described by Lebrun et al.
(1996), and terminated by the 3′ untranslated region of the histone
H4 gene of Arabidopsis
thaliana (Chabouté et al., 1987).
The hppdPf W336 gene coding sequence is under the control of the
sequence including the
promoter region of the Cassava Vein Mosaic Virus (CVMV; Verdaguer
et al., 1996), and by
the optimized transit peptide as described by Lebrun et al. (1996),
and terminated by the 3′
untranslated region of the histone H4 gene of Arabidopsis thaliana
(Chabouté et al., 1987).