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Modified Organism
BCS-GH811-4
Record information and status
Record ID
113966
Status
Published
Date of creation
2018-06-25 07:41 UTC (tim.strabala@epa.govt.nz)
Date of publication
2018-10-31 14:56 UTC (melissa.willey@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

Transformation event
GHB811 [DRAFT]
Unique identifier
BCS-GH811-4
Developer(s)
Bayer CropScience
Description
Cotton (Gossypium hirsutum) modified with resistance genes for the herbicides isoxaflutole and glyphosate via Agrobacterium-mediated transformation using the vector pTSIH09 containing the hppdPfW336-1Pa and 2mepsps expression cassettes. Specifically,

(i) The double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mepsps) gene
that encodes for the 2mEPSPS protein. The 2mepsps coding sequence was
developed by introducing two point mutations to the wild-type epsps gene cloned from
maize (Zea mays).
(ii) The hppdPf W336 gene encodes for the HPPD W336 protein. The hppdPf W336
coding sequence was developed by introducing a single point mutation to the wild type
hppd gene derived from Pseudomonas fluorescens. Expression of the HPPD W336
protein confers tolerance to isoxaflutole herbicides.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Related LMOs
BCS-GHØØ2-5 - GlyTol™ Cotton GHB614
Bayer CropScience Resistance to herbicides - Glyphosate
Show detection method(s)
MST-FGØ72-2 - Soy modified for tolerance to glyphosate and HPPD inhibitors
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
pTSIH09
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Histone H4 gene Promoter - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
5-enolpyruvylshikimate-3-phosphate synthase - Zea mays - Maize, Corn, MAIZE
Resistance to herbicides - Glyphosate
Histone H3 Gene II intron 1 - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Optimized Transit Peptide
Histone H4 gene 3' UTR - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
CsVMV promoter - Cassava vein mosaic virus - Cassava vein mosaic virus, CVMV, CsVMV
4-Hydroxyphenylpyruvate dioxygenase gene - Pseudomonas fluorescens - PSEFL
Resistance to herbicides
Notes regarding the genetic elements introduced or modified in this LMO
In the 2mepsps gene expression cassettes, the 2mepsps gene coding sequence is under
the control of the H4 promoter of Arabidopsis thaliana (Ph4A748; Chabouté et al., 1987),
followed by the first intron of gene II of the histone H3.III variant of Arabidopsis thaliana
(Chaubet et al., 1992) and by the optimized transit peptide as described by Lebrun et al.
(1996), and terminated by the 3′ untranslated region of the histone H4 gene of Arabidopsis
thaliana (Chabouté et al., 1987).
The hppdPf W336 gene coding sequence is under the control of the sequence including the
promoter region of the Cassava Vein Mosaic Virus (CVMV; Verdaguer et al., 1996), and by
the optimized transit peptide as described by Lebrun et al. (1996), and terminated by the 3′
untranslated region of the histone H4 gene of Arabidopsis thaliana (Chabouté et al., 1987).
LMO characteristics
Modified traits
Common use(s)
  • Food