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Modified Organism
GOR-7324Ø-2 - Safflower modified for altered fatty acid content
Record information and status
Record ID
113969
Status
Published
Date of creation
2018-08-19 23:52 UTC (gillian.colebatch@health.gov.au)
Date of last update
2019-02-08 21:51 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-02-08 21:51 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Safflower modified for altered fatty acid content
Transformation event
Event 40
Unique identifier
GOR-7324Ø-2
Developer(s)
Dr Carl Ramage
Regulatory and Compliance Manager
GO Resources Pty Ltd (GOR)
15 Sutherland Street
Brunswick
Melbourne, Victoria
Australia, 3056
Phone:+61 466642679
Email:carl@rautakisolutions.com.au
Description
The modified safflower contains RNAi gene silencing constructs that target two endogenous safflower fatty acid biosynthesis genes (CtFATB and CtFAD2.2), which are involved in the conversion of oleic acid to linoleic acid or palmitic acid. The gene silencing constructs suppress expression of the genes. As a result, the modified safflower produces seeds that accumulate a high proportion of oleic acid (approximately 92%) and very low linoleic acid (less than 2%). This high purity oleic acid has applications as an industrial raw material.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Carthamus tinctorius - Safflower, CARTI
Related LMOs
GOR-73226-6 - Safflower modified for altered fatty acid content (SHOSO26)
Dr Carl Ramage Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to antibiotics - Hygromycin
Characteristics of the transformation process
Vector
pCW732
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Linin gene promoter
2.03 Kb
 
 
Palmitoyl-Acyl Carrier Protein thioesterase
0.41 Kb
 
 
delta(12)-fatty acid desaturase
0.73 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.74 Kb
 
 
Catalase 1 intron
0.20 Kb
 
 
delta(12)-fatty acid desaturase
0.73 Kb
 
 
Palmitoyl-Acyl Carrier Protein thioesterase
0.41 Kb
 
 
Octopine Synthase Gene Terminator
0.74 Kb
 
 
CaMV 35S promoter
0.46 Kb
 
 
Hygromycin B phosphotransferase gene
1.24 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNA silencing construct
Transcription of the RNA silencing construct occurs from the Linum usitatissimum Linin gene promoter. The transcription of the Carthamus tinctorius palmitoyl-acyl carrier protein thioesterase (CtFATB) and delta(12)-fatty acid desaturase (CtFAD2.2) fragments occur first in the sense and then in the antisense orientation, separated by a spacer of Flaveria trinervia pyruvate orthophosphate dikinase intron (sense orientation) and Ricinus communis catalase 1 inton (in antisense orientation). Transcription terminates at the Agrobacterium tumefaciens octopine synthase gene terminator. The resulting transcript will form a hairpin structure (hairpin RNA - hpRNA) through the complemetary base pairing of sense and antisense sections of CtFATB and CtFAD2.2 and a loop of PDK int-1 and Cat-1. This structure can elicit RNA interference-mediated gene silencing (see below).

Seletion marker
The transcription of the Streptomyces sp. hygromycin B phosphotransferase gene occurs from the Cauliflower mosaic virus 35S promoter and terminates at the A. tumefaciens nopaline synthase gene terminator. Hygromycin B was used as a selectible marker for successful transformants during the development of the plant line.

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Note:
i) Only a portion of the full sequence was cloned into the gene silencing construct for both CtFATB and CtFAD2.2.
ii) Primer walking and southern blot analysis confirmed the presence of a single T-DNA insert into the safflower genome. The insertion of T-DNA generated a 34-basepair deletion and 35-basepair duplication within the genomic region. Truncated sequences of the Left Border (16bp) and Right Border (39 bp) were inserted into the genome.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Palmitoyl-Acyl Carrier Protein thioesterase - Carthamus tinctorius - Safflower, CARTI
Changes in quality and/or metabolite content - Lipid and fatty acids
delta(12)-fatty acid desaturase - Carthamus tinctorius - Safflower, CARTI
Changes in quality and/or metabolite content - Lipid and fatty acids
How the expression of the gene(s) was affected
RNA interference-mediated gene silencing
The double strandedness of the hpRNA triggers RNA interference in the plant cell. Essentially, specific plant proteins recognize and fragment the hpRNA into short interfering RNAs (siRNAs). These fragments are then used by plant cell proteins to specifically degrade mRNA transcripts with complementary sequences. In this case, the hpRNA causes the destruction of the CtFatb and CtFad2.2 mRNAs, preventing protein translation, and silencing gene expression.
Common use(s)
  • Research
  • Biofuel
  • Industrial oil

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication2 records
Modified Organism1 record
Risk Assessment2 records