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Modified Organism
MON-887Ø2-4 - Insect-protected cotton
Record information and status
Record ID
114159
Status
Published
Date of creation
2018-11-14 00:06 UTC (tim.strabala@epa.govt.nz)
Date of last update
2019-02-08 16:14 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-02-08 16:14 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect-protected cotton
Transformation event
MON88702
Unique identifier
MON-887Ø2-4
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Cotton modified to express the modified crystal protein mCry51Aa2 from Bacillus thuringiensis strain EG2934 to confer protection against hemipteran and thysanopteran insect pests, particularly Lygus hesperus and Lygus lineolaris.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Point of collection or acquisition of the recipient organism
Cotton cultivar DP 393
Characteristics of the transformation process
Vector
PV-GHIR508523
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S Enhancer
0.41 Kb
 
 
Heat shock protein 81-2 promoter
1.01 Kb
 
 
mCry51Aa2
0.92 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transcription of mCry51Aa2 coding sequence directed by the Arabidopsis thaliana Hsp81-2 promoter, with enhanced activity due to the Figwort mosaic virus 35S enhancer (E-FMV) located adjacent to the promoter in the 5' direction. Transcription is terminated by the Cauliflower mosaic virus (CaMV) 35S terminator.

Next-generation sequencing confirmed the vector backbone was not incorporated into the plant and the presence of a single T-DNA integration site*.

MCry51Aa2 is a 302 amino acid, 33.6kDa single polypeptide. This modified protein contains eight amino acid substitutions(F46S, Y54H, S95A, F147S, Q149E, S167R, P219R, R273W) and a deletion of three amino
acids (Δ196-198), which improve the effectiveness of the protein against the major Hemipteran cotton pests Lygus hesperus and Lygus lineolaris.  Immunoblotting of leaf samples and enzyme-linked immunosorbent assay (ELISA) of leaf, pollen, root, and seed samples confirmed the expression of the protein.

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Footnote:
* The plasmid PV-GHIR508523 contained two T-DNA regions:
1) E-FMV, P-Hsp81-2, mCry51Aa2, and T-CaMV
2) E-FMV, P-EF-1α (Elongation Factor 1 alpha promoter), TS-CTP2 (N-terminal chloroplast peptide), aadA (Escherichia coli streptomycin 3''-adenylyltransferase), and T-E9 (Pisum sativum rbcS-E9 gene terminator).
The T-DNAII was crossed out due to selective breeding and was not present in the final cotton line.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record