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Modified Organism
MON-87427-7 × MON-89Ø34-3 × DAS-Ø15Ø7-1 × MON-88Ø17-3 - Insect resistant, Herbicide tolerant Maize
Record information and status
Record ID
114244
Status
Published
Date of creation
2019-02-01 17:13 UTC (austein.mcloughlin@cbd.int)
Date of last update
2019-02-06 15:28 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-02-06 15:28 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant, Herbicide tolerant Maize
Transformation event
MON87427 × MON89034 × TC1507 × MON88017
Unique identifier
MON-87427-7 × MON-89Ø34-3 × DAS-Ø15Ø7-1 × MON-88Ø17-3
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
The stacked maize line was obtained through the traditional cross breeding of the parental organisms MON87427, MON89034, MON88017, and DAS01507. The line inherited genes that express cry1A.105, cry2Ab2,  cry3Bb1, cry1F, pat, and cp4epsps. The expression of these genes confer phosphinothricin (PPT), specifically glufosinate ammonium, and glyphosate herbicide tolerance, as well as resistance to Lepidoptera and Coleoptera pests.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-87427-7 - Maize modified for tissue selective glyphosate tolerance
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-89Ø34-3 - YieldGard™ VT Pro™
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMAP1043, PV-ZMIR245,  PV-ZMIR39,  PHI8999A (derived from plasmid PHP8999)
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid left border repeat
0.24 Kb
 
 
CaMV Enhanced 35S promoter
0.30 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry1A.105
3.53 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
 
FMV 35S promoter
0.56 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Transit peptide and first intron of Rubisco SSU
0.40 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid right border repeat
0.23 Kb
 
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.00 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
Rice actin 1 gene promoter
0.93 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.07 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Cry3Bb1
1.96 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.23 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON87427, vector PV-ZMAP1043:
MON87427 was modifies to express the CP4 EPSPS protein which confers tolerance to the herbicide glyphosate. The e35S-hsp70 promoter and intron combination is used to drive the tissue selective expression of the cp4 epsps gene resulting in CP4 EPSPS protein production in vegetative and female reproductive tissue, providing tolerance to glyphosate within these tissues.

DNA insert from MON89034 vector PV-ZMIR245:
Maize line MON89034 expresses two Bt-toxins encoded by the genes cry1A.105 and cry2Ab2 from Bacillus thuringiensis that confer resistance against certain lepidopteran pests.

DNA insert from DAS1507 vector PHI8999A:
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for cry1F, conferring resistance to European corn borer (Ostrinia nubilalis), and pat for glufosinate herbicide tolerance. The coding sequence of both genes has been optimized to achieve a high level of expression in maize. A leucine residue was modified (replacing a phenylalanine at position 604 of 605 amino acids in total) of Cry1F to create a specific restriction site for cloning purposes.

DNA insert from MON88017 vector PV-ZMIR39:
MON88017 expresses the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein to control infestation with corn root worm, and the cp4 epsps gene.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record